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nir1, a conditional-lethal mutation in barley causing a defect in nitrite reduction

Molecular and General Genetics MGG volume 236, pages 275–282 (1993)Cite this article

Summary

Eleven green individuals were isolated when 95000 M2 plants of barley (Hordeum vulgare L.), mutagenised with azide in the M1, were screened for nitrite accumulation in their leaves after nitrate treatment in the light. The selected plants were maintained in aerated liquid culture solution containing glutamine as sole nitrogen source. Not all plants survived to flowering and some others that did were not fertile. One of the selected plants, STA3999, from the cultivar Tweed could be crossed to the wild-type cultivar and analysis of the F2 progeny showed that leaf nitrite accumulation was due to a recessive mutation in a single nuclear gene, which has been designated Nir1. The homozygous nir1 mutant could be maintained to flowering in liquid culture with either glutamine or ammonium as sole nitrogen source, but died within 14 days after transfer to compost. The nitrite reductase cross-reacting material seen in nitrate-treated wild-type plants could not be detected in either the leaf or the root of the homozygous nir1 mutant. Nitrite reductase activity, measured with dithionite-reduced methyl viologen as electron donor, of the nitrate-treated homozygous nir1 mutant was much reduced but NADH-nitrate reductase activity was elevated compared to wild-type plants. We conclude that the Nir1 locus determines the formation of nitrite reductase apoprotein in both the leaf and root of barley and speculate that it represents either the nitrite reductase apoprotein gene locus or, less likely, a regulatory locus whose product is required for the synthesis of nitrite reductase, but not nitrate reductase. Elevation of NADH-nitrate reductase activity in the nir1 mutant suggests a regulatory perturbation in the expression of the Narl gene.

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  1. Dennis W. Kirk

    Present address: Department of Genetics and Microbiology, School of Life Sciences, University of Liverpool, P.O. Box 147, L69 3BX, Liverpool, UK

Affiliations

  1. Division of Environmental and Evolutionary Biology, School of Biological and Medical Sciences, Sir Harold Mitchell Building, University of St. Andrews, St. Andrews, Fife KY16 9TH, Scotland, UK

    Euan Duncanson, Amanda F. Gilkes, Dennis W. Kirk, Adrian Sherman & John L. Wray

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  1. Euan Duncanson
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  2. Amanda F. Gilkes
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  3. Dennis W. Kirk
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  4. Adrian Sherman
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  5. John L. Wray
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Communicated by H. Böhme

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Duncanson, E., Gilkes, A.F., Kirk, D.W. et al. nir1, a conditional-lethal mutation in barley causing a defect in nitrite reduction. Molec. Gen. Genet. 236, 275–282 (1993). https://doi.org/10.1007/BF00277123

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  • DOI: https://doi.org/10.1007/BF00277123

Key words

  • Hordeum vulgare L.
  • Nitrite reduction
  • Mutation