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The bacterial attachment site of the temperate Rhizobium phage 16-3 overlaps the 3′ end of a putative proline tRNA gene

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Abstract

Bacteriophage 16-3 inserts its genome into the chromosome of Rhizobium meliloti strain 41 (Rm41) by site-specific recombination. The DNA regions around the bacterial attachment site (attB) and one of the hybrid attachment sites bordering the integrated prophage (attL) were cloned and their nucleotide sequences determined. We demonstrated that the 51 by region, where the phage and bacterial DNA sequences are identical, is active as a target site for phage integration. Furthermore it overlaps the 3′ end of a putative proline tRNA gene. This gene shows 79% similartiy to the corresponding proline tRNA-like genomic target sequence of certain integrative plasmids in Actinomycetes.

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Authors and Affiliations

  1. Agricultural Biotechnology Center, Institute for Molecular Genetics, H-2100, Gödöllô Szent-Györgyi A u. 4, Hungary

    István Papp, Ferenc Olasz & László Orosz

  2. Department of Genetics, József Attila University, Középfasor 52, H-6726, Szeged, Hungary

    László Dorgai, Péter Papp, Ferenc Olasz & László Orosz

  3. Institute of Biochemistry, Biological Research Center of Hungarian Academy of Sciences, Temesvári krt. 62, H-6726, Szeged, Hungary

    Erzsébet Jónás

Authors
  1. István Papp
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  2. László Dorgai
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  3. Péter Papp
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  4. Erzsébet Jónás
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  5. Ferenc Olasz
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  6. László Orosz
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Communicated by A. Kondorosi

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Papp, I., Dorgai, L., Papp, P. et al. The bacterial attachment site of the temperate Rhizobium phage 16-3 overlaps the 3′ end of a putative proline tRNA gene. Molec. Gen. Genet. 240, 258–264 (1993). https://doi.org/10.1007/BF00277064

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  • DOI: https://doi.org/10.1007/BF00277064

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