Abstract
We have isolated from rice suspension cells three non-sequence-related cDNAs the expression of which is markedly induced by low, non-freezing temperature. Here we further characterize one of the cDNA clones, lip19. Expression of lip19 is positively regulated by low temperature, but not affected by high (40° C) temperature. Sequencing and primer extension analyses showed that lip19 has a long (552 bp) 5′ non-coding sequence followed by a single open reading frame specifying a protein of 148 amino acids. The deduced amino acid sequence of the protein, Lip19, shows at its amino-terminus a conserved basic region followed by a “leucine-zipper” domain. The reported sequence most similar to Lip19 is maize OCSBF-1, which is a bZip-type DNA binding protein. The possibility is suggested that Lip19 is a transcriptional factor that is positively controlled by low temperature.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aguan K, Sugawara K, Suzuki N, Kusano T (1991) Isolation of genes for low-temperature-induced proteins in rice by a simple subtractive method. Plant Cell Physiol 32:1285–1289
Gilmartin PM, Sarokin L, Memelink J, Chua NH (1990) Molecular light switches for plant genes. Plant Cell 2:369–378
Gilmour SJ, Artus NN, Thomashow MF (1992) cDNA sequence analysis and expression of two cold-regulated genes of Arabidopsis thaliana. Plant Mol Biol 18:13–21
Gruissem W (1990) Of fingers, zippers, and boxes. Plant Cell 2:827–828
Guiltinan MJ, Marcotte WR Jr, Quatrano RS (1990) A plant leucine zipper protein that recognizes an abscisic acid response element. Science 250:267–271
Guy CL, Niemi KJ, Brambl R (1985) Altered gene expression during cold acclimation of spinach. Proc Natl Acad Sci USA 82:3673–3677
Hartings H, Maddaloni M, Lazzaroni N, DiFonzo N, Motto M, Salamini F, Thompson R (1989) The O2 gene which regulates zein deposition in maize endosperm encodes a protein with structural homologies to transcriptional activators. EMBO J 8:2795–2801
Jones PG, Krah R, Tafuri SR, Wolffe AP (1992) DNA gyrase, CS7.4, and the cold shock response in Escherichia coli. J Bacteriol 174:5798–5802
Kao HT, Nevins JR (1983) Transcriptional activation and subsequent control of the human heat shock gene during adenovirus infection. Mol Cell Biol 3:2058–2065
Katagiri F, Lam E, Chua NH (1989) Two tobacco DNA-binding proteins with homology to the nuclear factor CREB. Nature 340:727–730
Katagiri F, Yamazaki K, Horikoshi M, Roeder RG, Chua NH (1990) A plant DNA-binding protein increases the number of active preinitiation complexes in a human in vitro transcription system. Genes Dev 4:1899–1909
Kurkela S, Franck M (1990) Cloning and characterisation of a cold- and ABA-inducible Arabidopsis gene. Plant Mol Biol 15:137–144
Kusano T, Aguan K, Abe M, Sugawara K (1992) Nucleotide sequence of a rice rab16 homologue gene. Plant Mol Biol 18:127–129
La Teana A, Brandi A, Falconi M, Spurio R, Pon CL, Gualerzi CO (1991) Identification of a cold shock transcriptional enhancer of the Escherichia coli gene encoding nucleoid protein H-NS. Proc Natl Acad Sci USA 88:10907–10911
Landschulz WH, Johnson PF, McKnight SL (1988) The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins. Science 240:1759–1763
Lin C, Thomashow MF (1992) A cold-regulated Arabidopsis gene encodes a polypeptide having potent cryoprotective activity. Biochem Biophys Res Commun 183:1103–1108
Lipman DJ, Pearson WR (1985) Rapid and sensitive protein similarity searches. Science 227:1435–1441
Lohmer S, Maddaloni M, Motto M, Di Fonzo N, Hartings H, Salamini F, Thompson RD (1991) The maize regulatory locus Opaque-2 encodes a DNA-binding protein which activates the transcription of the b-32 gene. EMBO J 10:617–624
McElroy D, Rothenberg M, Reece KS, Wu R (1990) Characterization of the rice (Oryza sativa) actin gene family. Plant Mot Biol 15:257–268
Mitchell PJ, Tjian R (1989) Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins. Science 245:371–378
Nagy F, Kay SA, Chua NH (1988) Analysis of gene expression in transgenic plants. In: Gelvin SB, Schilperoort RA (eds) Plant molecular biology manual, B4 Kluwer, Dordrecht, pp 1–29
Oeda K, Salinas J, Chua NH (1991) A tobacco bZip transcription activator (TAF-1) binds to a G-box-like motif conserved in plant genes. EMBO J 10:1793–1802
Sachs MM, Ho TD (1986) Alteration of gene expression during environmental stress in plants. Annu Rev Plant Physiol 37:363–376
Saiki RK, Gelfand DH, Stoffe S, Sharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Sano H, Youssefian S (1991) A novel ras-related rgp1 gene encoding a GTP-binding protein has reduced expression in 5-azacytidine-induced dwarf rice. Mol Gen Genet 228:227–232
Schindler U, Cashmore AR (1990) Photoregulated gene expression may involve ubiquitous DNA binding proteins. EMBO J 9:3415–3427
Schindler U, Menkens AE, Beckman H, Ecker JR, Cashmore AR (1992a) Heterodimerization between light-regulated and ubiquitously expressed Arabidopsis GBF bZIP protein. EMBO J 11:1261–1273
Schindler U, Terzaghi W, Beckmann H, Kadesch T, Cashmere AR (1992b) DNA binding site preferences and transcriptional activation properties of the Arabidopsis transcription factor GBF1. EMBO J 11:3415–3427
Schmidt RJ, Burr FA, Aukerman MJ, Burr B (1990) Maize regulatory gene opaque-2 encodes a protein with a “leucine-zipper” motif that binds to zein DNA. Proc Natl Acad Sci USA 87:46–50
Schmidt RJ, Ketudat M, Aukerman MJ, Hoschek G (1992) Opaque-2 is a transcriptional activator that recognizes a specific target site in 22-kD zein genes. Plant Cell 4:689–700
Singh K, Dennis ES, Ellis JG, Llewellyn DJ, Tokuhisa JG, Wahleithner JA, Peacock WJ (1990) OCSBF-1, a maize ocs enhancer binding factor: Isolation and expression during development. Plant Cell 2:891–903
Tabata T, Takase H, Takayama S, Mikami K, Nakatsuka A, Kawata T, Nakayama T, Iwabuchi M (1989) A protein that binds to a cis-acting element of wheat histone genes has a leucine zipper motif. Science 245:965–967
Tabata T, Nakayama T, Mikami K, Iwabuchi M (1991) HBP-1a and HBP-1b: leucine zipper-type transcription factors of wheat. EMBO J 10:1459–1467
Tabor S, Richardson CC (1985) A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc Natl Acad Sci USA 82:1074–1078
Tanabe H, Goldstein J, Yang M, Inouye M (1992) Identification of the promoter region of the Escherichia coli major cold shock gene, cspA. J Bacteriol 174:3867–3873
Thomashow MF (1990) Molecular genetics of cold acclimation in higher plants. Adv Genet 28:99–131
Weising K, Kahl G (1991) Towards an understanding of plant gene regulation: The action of nuclear factors. Z Naturforsch 46c:1–11
Weisshaar B, Armstrong GA, Block A, da Costa e Silva O, Hahlbrock K (1991) Light-inducible and constitutively expressed DNA-binding proteins recognizing a plant promoter element with functional relevance in light responsiveness. EMBO J 10:1777–1786
Wistow G (1990) Cold shock and DNA binding. Nature 344:823–824
Author information
Authors and Affiliations
Additional information
Communicated by H. Saedler
The sequence reported in this paper has been deposited in the EMBL/GenBank/DDBJ data base (Accession No. X57325)
Rights and permissions
About this article
Cite this article
Aguan, K., Sugawara, K., Suzuki, N. et al. Low-temperature-dependent expression of a rice gene encoding a protein with a leucine-zipper motif. Molec. Gen. Genet. 240, 1–8 (1993). https://doi.org/10.1007/BF00276876
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00276876