Abstract
Formation of gas and of methylated sulfur compounds was observed in anaerobic enrichment cultures with methoxylated aromatic compounds as substrates. Via direct dilution of mud samples in defined reduced media supplemented with trimethoxybenzoate or syringate two new strains of anaerobic homoacetogenic bacteria (strain TMBS4 and strain SA2) were obtained in pure culture. Both strains produced dimethylsulfide and methanethiol during growth on methoxylated aromatic compounds. Growth tests and determination of stoichiometries demonstrated that the volatile sulfur compounds were formed from the methyl group at the aromatic ring and the sulfide added as reducing agent to the medium (R = aromatic residue): 2 R - O - CH3 + H2 S → 2 R - OH + (CH3)2S
Dimethylsulfide was the major organic sulfur compound formed, whereas methanethiol appeared only as intermediate in small quantities. The isolates grew also with trihydroxybenzenes such as gallate, phloroglucinol, or pyrogallol without formation of methylated sulfur compounds. The aromatic compounds were degraded to acetate. The freshwater strain TMBS4 also fermented pyruvate. Other aliphatic or aromatic compounds were not utilized. External electron acceptors (sulfate, nitrate, fumarate) were not reduced. Both strains were mesophilic and formed rod-shaped, non-motile, Gram-negative cells. Spore formation was not observed. Tentatively, both isolates can be affiliated to the genus Pelobacter.
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Abbreviations
- TMB:
-
3,4,5-trimethoxybenzoate
- MT:
-
methanethiol
- DMS:
-
dimethylsulfide
References
Aneja VP, Cooper WJ (1987) Biogenic sulfur emissions: a review. In: Saltzman ES, Cooper WJ (eds) Biogenic sulfur in the environment. ACS, Washington, pp 2–14
Andreae MO (1986) The ocean as a source of atmospheric sulfur compounds. In: Buat-Menard P (ed) The role of air-sea exchange in geochemical cycling. Reidel, New York, pp 331–362
Bache R, Pfennig N (1981) Selective isolation of Acetobacterium woodii on methoxylated aromatic acids and determination of growth yields. Arch Microbiol 147: 184–189
Brune A, Schink B (1990) Pyrogallol to phloroglucinol conversion and other hydroxyl-transfer reactions catalyzed by cell extracts of Pelobacter acidigallici. J Bacteriol 172: 1070–1076
Daniel SL, Wu Z, Drake HL (1988) Growth of thermophilic acetogenic bacteria on methoxylated aromatic acids. FEMS Microbiol Lett 52: 25–28
DeWeerd KA, Saxena A, Nagle DP, Suflita JU (1988) Metabolism of the 18O-methoxy substituent of 3-methoxybenzoic acid by anaerobic bacteria. Appl Environ Microbiol 54: 1237–1242
Drotar A, Burton GA, Tavernier JE, Fall R (1987a) Widespread occurrence of bacterial thiol methyltransferases and the biogenic emissions of methylated sulfur gases. Appl Environ Microbiol 53: 1626–1631
Drotar A, Fall LR, Mishalanie EA, Travernier JE, Fall R (1987b) Enzymatic methylation of sulfide, selenite, and organic thiols by Tetrahymena thermophila. Appl Environ Microbiol 53: 2111–2118
Encyclopedie des gaz (1976) Methyl mercaptane. Elsevier, New York, pp 305–310
Finster K, King GM, Bak F (1990) Formation of methylmercaptan and dimethylsulfide from methoxylated aromatic compounds in anoxic marine and freshwater sediments. FEMS Microbiol Ecol 74: 295–302
Hyninen P (1971) On the production of methyl mercaptan and its oxidation products in air or steam stripping of sulfate mill condensates. Paper och trä. Specialnummer 4a: 159–169
Kadota H, Ishida Y (1972) Production of volatile sulfur compounds by microorganisms. Annu Rev Microbiol 26: 127–138
Kelly DP, Smith NA (1990) Organic sulfur compounds in the environment. In: Marshall KC (ed) Advances in microbial ecology, vol 11. Plenum Press, New York, London, pp 345–385
Kiene RP, Capone DG (1988) Microbial transformations of methylated sulfur compounds in anoxic salt marsh sediments Microb Ecol 15: 275–291
Lovelock JE, Maggs RJ, Rasmussen RA (1972) Atmospheric dimethyl sulfide and the natural sulphur cycle. Nature 237: 452–453
Möller D (1984) On the global natural sulfur emissions. Atoms Environ 18: 29–39
Schink B, Pfennig N (1982) Fermentation of trihydroxybenzenes by Pelobacter acidigallici gen. nov. sp. nov., a new strictly anaerobic, non-sporeforming bacterium. Arch Microbiol 133: 195–201
Taylor BF, Kiene RP (1989) Microbial metabolism of dimethyl sulfide in the oceans. In: Saltzman ES, Cooper WJ (eds) Biogenic sulfur in the environment. ACS, Washington, pp 202–221
Vairavamurthy A, Mopper K (1987) Geochemical formation of organosulphur compounds (thiols) by addition of H2S to sedimentary organic matter. Nature 329: 623–625
Widdel F, Bak F (1991) Gram-negative mesophilic sulfate-reducing bacteria. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH (eds) The prokaryotes, 2nd edn, vol 4. Springer, New York, Berlin Heidelberg, pp 3352–3378
Wu Z, Daniel SL, Drake HL (1988) Characterization of a CO-dependent O-demethylating enzyme system from the acetogen Clostridium thermoaceticum. J Bacteriol 170: 5747–5750
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Bak, F., Finster, K. & Rothfuß, F. Formation of dimethylsulfide and methanethiol from methoxylated aromatic compounds and inorganic sulfide by newly isolated anaerobic bacteria. Arch. Microbiol. 157, 529–534 (1992). https://doi.org/10.1007/BF00276773
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DOI: https://doi.org/10.1007/BF00276773