Abstract
Bone remodelling was studied in allografts and isografts by means of autoradiography. Collagen and proteglycanes of the organic bone matrix were labelled with H-3 aminoacids and S-35 sodium sulphate. The principles of primary bone formation are followed in graft remodelling as well. Matrix breakdown and reformation are carried out by mononuclear cells. Components of the original matrix can be seen in osteoclasts, perivascular cells, osteoblasts, young osteocytes, and in new bone. The label allows tracing of these components and proves their reuse.
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References
Carneiro J (1965) Synthesis and turnover of collagen in periodontal tissues. In: Leblond CP (ed) The use of radioautography in investigating protein synthesis. Academic Press, New York, London
Fonseca RJ, Nelson JF, Clark PJ, Frost DE, Olson RA (1983) Revascularization and healing of onlay particulate allogenic bone grafts in primates. J Oral-maxillofac Surg 40:153–162
Frost DE, Fonseca RJ, Burkes EJ (1982) Healing of interpositional allogenic lyophilized bone grafts following total maxillary osteotomy. J Oral Maxillofac Surg 40:776–786
Greulich RG, Slavkin HC (1965) Amino acid utilization in the synthesis of enamel and dentin matrices as visualized in autoradiography. In: Leblond CP (ed) The use of autoradiography in investigating protein synthesis. Academic Press, New York London
Hildmann H, Koburg E, Steinbach E (1972) Über das Schicksal cialitkonservierter Homoiotransplantate im Mittelohr. Arch Ohren Nasen Kehlk Heilk 207:666–670
Hildmann H, Steinbach E, Koburg E (1974) The fate of bone transplants in the middle ear. J Laryngol Otol 88:531–537
Hildmann H, Hettich R (1975) An autoradiographic study on bone new formation in isografts. Paper presented at the 10th Congr Eur Soc Exp Surg (Paris)
Hildmann H (1976) Tierexperimentelle Modelluntersuchung zur Verkleinerung von Radikaloperationshöhlen mit homologem konserviertem Knochengewebe. Laryngol Rhinol Otol 55:812–816
Hildmann H (1982) Isologe Transplantate im Mittelohr. Laryngol Rhinol Otol 61:26–29
Irving JT, Heeley JD (1970) Resorbtion of bone collagen by multinucleated cells. Calcif Tissue Res Suppl 6:254–259
Leblond CP, Weinstock M (1971) In: Bourne GH (ed) The biochemistry and physiology of bone. Academic Press, New York London
Niklas A, Oehlert W (1956) Grundsätzliches zum Zusammenhang zwischen autoradiografischer Schwärzung und dem Eiweißumsatz des betreffenden Gewebes bzw Zellarten. Beitr Pathol Anat 116:98–123
Ochlert W (1968) Orthologie und Pathologie des Schwefelstoffwechsels der Zelle im Autoradiogramm. In: Handbuch der allgemeinen Pathologie II. Springer, Berlin Heidelberg New York, pp 671–696
Rahn BA, Perren SM (1970) Calcein blue as a fluorescent label in bone. Experientia (Basel) 26:519
Rahn BA, Perren SM (1972) Alizarinkomplexon, ein neues Fluorochrom zur intravasalen Markierung des Knochenbaues. Experientia (Basel) 28:180
Tonna EA (1962) An autoradiographic examination of the utilization of tritiated histidine by cells of the skeletal system. Nature 193:1301–1302
Tonna EA (1965) Protein synthesis and cells of the skeletal system. In: Leblond CP (ed) The use of radioautography in investigation of protein synthesis. Academic Press, New York London, pp 215–245
Tonna EA (1971) An autoradiographic study of H3proline utilization by aging mouse skeletal tissues. Exp Gerontol 6:405–415
Tonna EA (1974) Zellkinetik des Knochens und des Alterns. Verh Dtsch Ges Pathol 58:99–102
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Hildmann, H., Tiedjen, K.U. & Hildmann, A. An autoradiographic study of bone grafts in small animals. Eur J Nucl Med 12, 147–150 (1986). https://doi.org/10.1007/BF00276708
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DOI: https://doi.org/10.1007/BF00276708