Abstract
Reticuloendothelial function and HLA type were studied in 22 controls and 15 patients with Wegener's granulomatosis (WG). IgG-coated red cells were injected intravenously and half-life times of blood disappearance and liver spleen uptake curves were related to the degree of antibody coating. Erythrocytes with 13,990 molecules of IgG per cell gave biexponential blood disapperance curves and were suitable for measuring splenic reticuloendothelial function. Half-life times thus obtained were not significantly different for individuals with the HLA-DR2 or DR3 phenotype. In WG patients with major disease activity, blood clearance of the injected cells was considerably decreased. Kinetic analysis of blood disappearance and spleen uptake curves revealed that this was partly due to a decrease in reversible trapping of the cells in the spleen. This suggests that a blocking effect of circulating immune complexes on splenic Fc receptors is not likely to be the sole cause of the observed hyposplenism.
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Elkon KB, Sewel JR, Ryan PFJ, Hughes GRV (1980) Splenic function in non-renal systemic lupus erythematosus. Am J Med 69:80–82
Elkon KB, Ferjencik PP, Walport MJ, Peters AH, Lewis SM, Hughes GFR (1982) Evaluation of heat-damaged and IgG-coated red cells for testing reticuloendothelial function. J Immunol Methods 55:253–263
Fehr J, Hofmann V, Kappeler U (1982) Transient reversal of thrombocytopenia in idiopathic thrombocytopenic purpura by high-dose intravenous gamma globulin. N Engl J Med 306:1254–1258
Frank MM, Hamburger MI, Lawley TJ, Kimberly RP, Plotz DH (1979) Defective reticuloendothelial system Fc receptor function in systemic lupus erythematosus. N Engl J Med 300:518–523
Frank MM, Lawley TJ, Hamburger MI, Brown EJ (1983) Immunoglobulin G Fc receptor-mediated clearance in autoimmune diseases. Ann Intern Med 98:206–218
Godman GC, Churg J (1954) Wegener's granulomatosis. Arch Pathol 58:533
Lockwood CM, Worlledge S, Nicholas A, Cotton Ch, Keith-Peters D (1979) Reversal of impaired splenic function in patients with nephritis or vasculitis (or both) by plasma exchange. N Engl J Med 300:524–530
Merry AH, Thomson EE, Rawlinson VI, Stratton F (1982) A quantitative antiglobulin test for IgG for use in blood transfusion serology. Clin Lab Haematol 4:393–402
Meryhew NL, Runquist OA (1981) A kinetic analysis of immunemediated clearance of erythrocytes. J Immunol 126:2443–2449
Mollison PL (1983) Blood transfusion in clinical medicine, 7th edn. Blackwell, Oxford, pp 588–594
Parris TM, Kimberley RP, Inman RD, McDougal JS, Gibofsky A, Cristian CL (1982) Defective Fc receptor-mediated function of the mononuclear phagocyte system in lupus nephritis. Ann Intern Med 97:526
Peters AM, Walport MJ, Elkon KB, Reavy HJ, Ferjencik PP, Lavender JP, Hughes GRV (1984) The comparative blood clearance kinetics of modified radio labelled erythrocytes. Clin Science 66:55–62
Shaw DR, Griffin FM (1981) Phagocytosis requires repeated triggering of macrophage phagocytic receptors during particle ingestion. Nature 289:409–411
Solomon LR, Rawlinson VI, Howard S, Mallick NP (1984) Reticuloendothelial function in glomerulonephritis. Nephron 37:54–59
van Rood JJ, van Leeuwen A, Ploem JS (1976) Simultaneous detection of two cell populations by two-colour fluorescence and application on the recognition of B-cell determinants. Nature 262:795–797
van der Woude FJ, Piers DA, van der Giessen M, Hoedemaeker PhJ, The TH, van der Hem GK (1983) Abnormal reticuloendothelial function in patients with active vasculitis and idiopathic membranous glomerulopathy. Eur J Nucl Med 8:60–64
van der Woude FJ, van der Giessen M, Kallenberg CGM, Ouwehand W, Beekhuis H, Beelen JM, van Son WJ, Hoedemaeker PhJ, van der Hem GK, The TH (1984) Reticuloendothelial Fc receptor function in SLE patients. I. Primary HLA linked defect or acquired dysfunction secondary to disease activity? Clin Exp Immunol 55:473–480
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van der Woude, F.J., Beekhuis, H., Beelen, J.M. et al. Kinetic analysis of the blood clearance and organ uptake curves of IgG-coated red cells in HLA-typed controls and patients with Wegener's granulomatosis. Eur J Nucl Med 12, 130–136 (1986). https://doi.org/10.1007/BF00276705
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DOI: https://doi.org/10.1007/BF00276705