Abstract
The role of the leukocyte isolation procedure on cell labelling with 99mTc has been evaluated. Separation of leukocytes was performed by two procedures: (1) sedimentation on methyl cellulose, followed by discontinuous gradient centrifugation; (2) methyl cellulose sedimentation and hypotonic haemolysis of residual red blood cells. After washing the cells in saline and incubation with a stannous pyrophosphate agent, the leukocytes were labelled with 5–10 mCi 99mTc. Procedure 1 gave a higher purity but lower recovery of polymorphonuclear leukocytes, and a minor contamination of red blood cells. 99mTc labelling of cells was slightly more efficient with this method, probably due to the presence of red blood cells. Procedure 1 is recommended for in vitro studies on cell kinetics and procedure 2 is recommended for clinical use.
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Kelbæk, H. Isolation and labelling of human leukocytes with 99mTc. Eur J Nucl Med 12, 107–109 (1986). https://doi.org/10.1007/BF00276700
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DOI: https://doi.org/10.1007/BF00276700