Summary
Purified DNA sequences that code for tRNA in Neurospora crassa were isolated and partially characterized. The tRNA cistrons comprise about 0.3 percent of the N. crassa genome. The tRNA:tDNA hybrids showed a buoyant density in cesium sulfate of 1.48 g cm-3 and sedimented in an intermediate position between native DNA and tRNA of N. crassa as expected. Te 50 of hybridized tRNA:tDNA molecules, reassociated tDNA: native DNA and homoduplexes of native DNA were 83.0°C, 88.5°C and 89.5°C respectively from thermal stability studies using hydroxyapatite chromatography. The isolated tRNA cistrons react almost completely with unlabeled DNA and tRNA of N. crassa or tRNA of the slime mutant of N. crassa; but react poorly with either ribosomal RNA or ribosomal RNA cistrons of N. crassa and tRNA of Escherichia coli. It appears that tRNA genes of N. crassa are repeated.
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Communicated by F. Kaudewitz
This research was supported from grants from U.S. Atomic Energy Commission No. At(40-1)-4182 and the Anna Fuller Fund, New Haven, Connecticut to S.K.D. we are grateful to Dr. J. White, Director of H.U. Cancer Res. Center for help.
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Dutta, S.K., Ray, R. Partial characterization of transfer RNA genes isolated from Neurospora crassa . Molec. Gen. Genet. 125, 295–300 (1973). https://doi.org/10.1007/BF00276585
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DOI: https://doi.org/10.1007/BF00276585