Summary
A mutant of Hansenula wingei, resistant to ethidium bromide (EB) on glucose medium, was analyzed for both meiotic and mitotic segregation. EB resistance on glucose was found to be recessive and due to mutation of two linked nuclear genes, called etb1 and etb2, which are separated by 18 centimorgans. A linked, pleiotropic, glycerol-negative gene (glp) increases the already high frequency of mitotic segregation of these EB resistant loci from the heterozygous diploid about 7-fold. Genetic analysis of six genes has defined only two linkage groups indicating that H. wingei has a small number of chromosomes. This is in agreement with cytological observations by C. Robinow which show that in H. wingei the haploid chromosome number is four.
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Crandall, M., Richter, R.H. Genetics of resistance to ethidium bromide in the petite-negative yeast Hansenula wingei . Molec. Gen. Genet. 125, 279–293 (1973). https://doi.org/10.1007/BF00276584
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DOI: https://doi.org/10.1007/BF00276584