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The suitability of restriction fragment length polymorphisms as genetic markers in maize

Theoretical and Applied Genetics volume 71, pages 765–771 (1986)Cite this article

Summary

Strain identification in Zea mays by restriction fragment length polymorphism should be feasible due to the high degree of polymorphism found at many loci. The polymorphism in maize is apparently higher than that currently known for any other organism. Five randomly selected maize inbred lines were examined by Southern filter hybridization with probes of cloned low copy sequences. Typically, several alleles could be distinguished among the inbred lines with any one probe and an appropriately selected restriction enzyme. Despite considerable polymorphism at the DNA level, 16 RFLP markers in three inbred lines of maize were examined for six to 11 generations and found be stable. Mapping of RFLP markers in maize can be accelerated by the use of B-A translocation stocks, which enable localization of a marker to chromosome arm in one generation. The use of recombinant inbred lines in further refinement of the map is discussed.

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Author notes

  1. S. V. Evola

    Present address: CIBA-GEIGY Corp., Biotechnology Research, P.O. Box 12257, 27709, Research Triangle Park, NC, USA

Affiliations

  1. Department of Biology, Brookhaven National Laboratory, 11973, Upton, NY, USA

    S. V. Evola, F. A. Burr & B. Burr

Authors
  1. S. V. Evola
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  2. F. A. Burr
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  3. B. Burr
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Communicated by A. L. Kahler

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Evola, S.V., Burr, F.A. & Burr, B. The suitability of restriction fragment length polymorphisms as genetic markers in maize. Theoret. Appl. Genetics 71, 765–771 (1986). https://doi.org/10.1007/BF00276416

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  • DOI: https://doi.org/10.1007/BF00276416

Key words

  • Zea mays L
  • Restriction fragment length polymorphism (RFLP)
  • Genetic mapping
  • B-A translocations
  • Recombinant inbreds