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Failure to document fetal cells in maternal circulation using the Selypes-Lorencz “air-culture” cytogenetic technique

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Summary

Isolating and analyzing fetal cells circulating in the maternal circulation would allow a relatively noninvasive method (i.e., venipuncture) for prenatal cytogenetic diagnosis. Several groups have claimed evidence for the presence of fetal cells in maternal circulation, and in one communication, Selypes and Lorencz (1988) reported the presence of relatively large numbers of fetal mitotic cells in phytohemagglutinin-stimulated blood cultures derived from pregnant women. We employed the laboratory method of Selypes and Lorencz in evaluating maternal blood from 29 patients (9.7 to 19.5 weeks gestation) in which the fetal complement differed from that of the mother (e.g., 46,XY, aneuploidy). Unfortunately, we were unable to confirm the results of Selypes and Lorencz.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, University of Tennessee at Memphis, 853 Jefferson Avenue, 38163, Memphis, TN, USA

    Mounira Youssef, Lee P. Shulman, Joe Leigh Simpson & Sherman Elias

  2. Department of Pediatrics, University of Tennessee at Memphis, 853 Jefferson Avenue, 38163, Memphis, TN, USA

    Avirachan T. Tharapel

  3. Ain Shams University Medical Genetics Center, Cairo, Egypt

    Mounira Youssef

Authors
  1. Mounira Youssef
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  2. Lee P. Shulman
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  3. Avirachan T. Tharapel
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  4. Joe Leigh Simpson
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  5. Sherman Elias
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Youssef, M., Shulman, L.P., Tharapel, A.T. et al. Failure to document fetal cells in maternal circulation using the Selypes-Lorencz “air-culture” cytogenetic technique. Hum Genet 85, 133–134 (1990). https://doi.org/10.1007/BF00276341

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  • DOI: https://doi.org/10.1007/BF00276341

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