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Direct estimation of serological H-Y antigen by flow cytometry

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Summary

This study was undertaken to evaluate the efficiency of flow cytometry in the detection of the serological H-Y antigen, and to survey expression of HY in the normal human population. Peripheral blood leukocytes (granulocytes) were reacted with monoclonal H-Y antibody, gw-16, and with FITC-conjugated goat anti-mouse IgG, and then were scored for fluorescence in the flow cytometer. Assigned H-Y phenotype was correlated accurately with sex phenotype in 33 out of 38 women and 62 out of 65 men. Data were evaluated by exploratory data analysis (EDA).

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Authors and Affiliations

  1. College of Agricultural and Life Sciences, University of Wisconsin, 53706-1284, Madison, WI, USA

    Marijo Kent

  2. Division of Reproductive Genetics, Department of Obstetrics and Gynecology, University of Tennessee at Memphis, 711 Jefferson Avenue, 38105, Memphis, TN, USA

    Stephen Wachtel

  3. Memorial Sloan-Kettering Cancer Center, 10021, New York, NY, USA

    H. Tzvi Thaler

Authors
  1. Marijo Kent
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  2. Stephen Wachtel
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  3. H. Tzvi Thaler
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Kent, M., Wachtel, S. & Thaler, H.T. Direct estimation of serological H-Y antigen by flow cytometry. Hum Genet 85, 127–129 (1990). https://doi.org/10.1007/BF00276339

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  • DOI: https://doi.org/10.1007/BF00276339

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