Skip to main content
Log in

The analysis of hypervariable DNA profiles: problems associated with the objective determination of the probability of a match

  • Original Investigations
  • Published:
Human Genetics Aims and scope Submit manuscript

Summary

A computerised system has been used to store DNA profiles from 3 hypervariable loci. This initial survey illustrates that band matching is only possible after analysis of the errors associated with electrophoretic systems. A number of databases have been constructed with the three probes investigated and two methods of frequency determination, ‘binning’ and ‘sliding window’ fitting, have been compared.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Baird M, Balazs I, Giusti A, Miyazaki L, Nicholas L, Wexler K, Kanter E, Glassberg J, Allen F, Rubinstein P, Sussman L (1986) Allele frequency distribution to two highly polymorphic DNA sequences in three ethnic groups and its application to the determination of paternity. Am J Hum Genet 29:489–501

    Google Scholar 

  • Bell GI, Selby MJ, Rutter WJ (1982) The highly polymorphic region near the human insulin gene is composed of simple tandemly repeating sequences. Nature 195:31–35

    Google Scholar 

  • Church GM, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81:1991–1995

    Google Scholar 

  • Elder JK, Southern EM (1987) Computer-aided analysis of one-dimensional restriction fragment gels. In: Bishop MJ, Rawlings CJ (eds) Nucleic acid and protein sequence analysis. IRL Press, Oxford, pp 165–172

    Google Scholar 

  • Evett IW, Werrett DJ, Gill P, Buckleton JS (1989) DNA fingerprinting on trial. Nature 340:435

    Google Scholar 

  • Gill P, Jeffreys AJ, Werrett DJ (1985) Forensic application of DNA ‘fingerprints’. Nature 318:577–579

    Google Scholar 

  • Gill P, Lygo JE, Fowler SJ, Werrett DJ (1987) An evaluation of DNA fingerprinting for forensic purposes. Electrophoresis 8:35–38

    Google Scholar 

  • Gjertson DW, Mickey MR, Hopfield J, Takenouchi T, Terasaki PI (1988) Calculation of probability of paternity using DNA sequences. Am J Hum Genet 43:860–869

    Google Scholar 

  • Jeffreys AJ, Wilson V, Thein SL (1985) Hypervariable ‘minisatellite’ regions in human DNA. Nature 314:67–73

    Google Scholar 

  • Lander ES (1989) DNA fingerprinting on trial. Nature 339:501–505

    Google Scholar 

  • Nakamura Y, Leppert M, O'Connell P, Wolff R, Holm T, Culver M, Martin C, Fujimoto E, Hoff M, Kumlin E, White R (1987) Variable number of tandem repeats (markers) for human gene mapping. Science 235:1616–1622

    Google Scholar 

  • Nakamura Y, Lathrop M, O'Connell P, Leppert M, Barker D, Wright M, Skolinick M, Konoleon S, Litt M, Lalouel JM, White R (1988) A mapped set of DNA markers for human chromosome 17. Genomics 2:302–309

    Google Scholar 

  • Proudfoot NJ, Gil A, Maniatis T (1982) The structure of the human beta-globin and a closely linkes, nearly identical pseudoene. Cell 31:553–563

    Google Scholar 

  • Wong Z, Wilson V, Patel I, Povey S, Jeffreys AJ (1987) Characterisation of a panel of highly variable minisatellites cloned from human DNA. Ann Hum Genet 51:269–288

    Google Scholar 

  • Wyman A, White R (1980) A highley polymorphic locus in human DNA. Proc Natl Acad Sci USA 77:6754–6758

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gill, P., Sullivan, K. & Werrett, D.J. The analysis of hypervariable DNA profiles: problems associated with the objective determination of the probability of a match. Hum Genet 85, 75–79 (1990). https://doi.org/10.1007/BF00276327

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00276327

Keywords

Navigation