Summary
A computerised system has been used to store DNA profiles from 3 hypervariable loci. This initial survey illustrates that band matching is only possible after analysis of the errors associated with electrophoretic systems. A number of databases have been constructed with the three probes investigated and two methods of frequency determination, ‘binning’ and ‘sliding window’ fitting, have been compared.
Similar content being viewed by others
References
Baird M, Balazs I, Giusti A, Miyazaki L, Nicholas L, Wexler K, Kanter E, Glassberg J, Allen F, Rubinstein P, Sussman L (1986) Allele frequency distribution to two highly polymorphic DNA sequences in three ethnic groups and its application to the determination of paternity. Am J Hum Genet 29:489–501
Bell GI, Selby MJ, Rutter WJ (1982) The highly polymorphic region near the human insulin gene is composed of simple tandemly repeating sequences. Nature 195:31–35
Church GM, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81:1991–1995
Elder JK, Southern EM (1987) Computer-aided analysis of one-dimensional restriction fragment gels. In: Bishop MJ, Rawlings CJ (eds) Nucleic acid and protein sequence analysis. IRL Press, Oxford, pp 165–172
Evett IW, Werrett DJ, Gill P, Buckleton JS (1989) DNA fingerprinting on trial. Nature 340:435
Gill P, Jeffreys AJ, Werrett DJ (1985) Forensic application of DNA ‘fingerprints’. Nature 318:577–579
Gill P, Lygo JE, Fowler SJ, Werrett DJ (1987) An evaluation of DNA fingerprinting for forensic purposes. Electrophoresis 8:35–38
Gjertson DW, Mickey MR, Hopfield J, Takenouchi T, Terasaki PI (1988) Calculation of probability of paternity using DNA sequences. Am J Hum Genet 43:860–869
Jeffreys AJ, Wilson V, Thein SL (1985) Hypervariable ‘minisatellite’ regions in human DNA. Nature 314:67–73
Lander ES (1989) DNA fingerprinting on trial. Nature 339:501–505
Nakamura Y, Leppert M, O'Connell P, Wolff R, Holm T, Culver M, Martin C, Fujimoto E, Hoff M, Kumlin E, White R (1987) Variable number of tandem repeats (markers) for human gene mapping. Science 235:1616–1622
Nakamura Y, Lathrop M, O'Connell P, Leppert M, Barker D, Wright M, Skolinick M, Konoleon S, Litt M, Lalouel JM, White R (1988) A mapped set of DNA markers for human chromosome 17. Genomics 2:302–309
Proudfoot NJ, Gil A, Maniatis T (1982) The structure of the human beta-globin and a closely linkes, nearly identical pseudoene. Cell 31:553–563
Wong Z, Wilson V, Patel I, Povey S, Jeffreys AJ (1987) Characterisation of a panel of highly variable minisatellites cloned from human DNA. Ann Hum Genet 51:269–288
Wyman A, White R (1980) A highley polymorphic locus in human DNA. Proc Natl Acad Sci USA 77:6754–6758
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gill, P., Sullivan, K. & Werrett, D.J. The analysis of hypervariable DNA profiles: problems associated with the objective determination of the probability of a match. Hum Genet 85, 75–79 (1990). https://doi.org/10.1007/BF00276327
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00276327