Summary
The directions of replication of several prophages integrated with a known orientation in the vicinity of the terminus (tre) of chromosome replication (trp::Mu, min 27; λrev integrated within rac, min 31, man::Mu, min 35), have been established by determining the molecular polarity of Okazaki pieces specific to these prophages. The results obtained strongly suggest that the site tre is located between rac and man, an otherwise genetically silent region.
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Communicated by B.A. Bridges
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Louarn, J., Patte, J. & Louarn, JM. Map position of the replication terminus on the Escherichia coli chromosome. Molec. Gen. Genet. 172, 7–11 (1979). https://doi.org/10.1007/BF00276208
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DOI: https://doi.org/10.1007/BF00276208