Summary
A recessive suppressor, su-1, of arg-2 was used to detect somatic recombination in common-AB diploids of S. commune. Recombinants were recovered from dense, fastgrowing sectors on arginine-deficient medium. The majority of spontaneous recombinants (129/154) recovered in this study were apparently haploid. Strains which scored as aneuploid and diploid were also recovered and analyzed. Genetic analysis of spontaneous recombinants indicated that crossing over is rare and that haploidization very likely proceeds via stages of aneuploidy. No increase in the frequency of crossing over was detected in recombinants derived following treatment with UV light. The preliminary results favor a parasexual mechanism of recombination in common-AB diploids.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cowan, J. W., Lewis, D.: Somatic recombination in the dikaryon of Coprinus lagopus. Genet. Res. 7, 235–244 (1966).
Crowe, L. K.: The exchange of genes between nuclei of a dikaryon. Heredity 15, 397–405 (1960).
Ellingboe, A. H.: Illegitimacy and specific factor transfer in Schizophyllum commune. Proc. nat. Acad. Sci. (Wash.) 49, 286–292 (1963).
—: Somatic recombination in dikaryon K of Schizophyllum commune. Genetics 49, 247–251 (1964).
—, Raper J. R.: Somatic recombination in Schizophyllum commune. Genetics 47, 85–98 (1962).
Käfer, E.: The process of spontaneous recombination in vegetative nuclei of Asperigillus nidulans. Genetics 46, 1581–1609 (1961).
Middleton, R. B.: Sexual and somatic recombination in common-AB heterokaryons of Schizophyllum commune. Genetics 50, 701–710 (1964)
Mills, D. I., Ellingboe, A. H.: Induction and characterization of recessive suppressors of arg-2 in Schizophyllum commune. Molec. Gen. Genetics 104, 313–320 (1969a).
—: A common-AB diploid of Schizophyllum commune. Genetics 62, 271–279 (1969b).
Papazian, H. P.: Exchange of incompatibility factors between the nuclei of a dikaryon. Science 119, 691–693 (1954).
Pontecorvo, G.: The parasexual cycle in fungi. Ann. Rev. Microbiol. 10, 393–400 (1956).
—, Käfer, E.: Genetic analysis based on mitotic recombination. Advanc. Genet. 9, 71–104 (1958).
Quintanilha, A.: Étude génétique du phénomène de Buller. Bol. Soc. Broter 13, 425–486 (1939).
Raper, J. R., Miles, P. G.: The genetics of Schizophyllum commune. Genetics 43, 530–546 (1958).
Roman, H.: Studies of gene mutation in Saccharomyces. Cold Spr. Harb. Symp. quant. Biol. 23, 111–118 (1956).
—: Gene conversion in fungi. In: Methodology in basic genetics, ed. by W. J. Burdette, p. 209–227. San Francisco: Holden-Day 1963.
Swiezynski, K. M.: Analysis of an incompatible di-mon mating in Coprinus lagopus. Acta Soc. Bot. Pol. 31, 169–184 (1962).
—: Somatic recombination of two linkage groups in Coprinus lagopus. Genet. Pol. 4, 21–36 (1963).
Wood, S., Käfer, E.: Effects of ultraviolet irradiation in heterozygous diploids of Aspergillus nidulans. I. UV-induced mitotic crossing over. Genetics 62, 507–518 (1969).
Author information
Authors and Affiliations
Additional information
Communicated by F. Kaudewitz
Journal Article No. 5126, Michigan Agricultural Experiment Station. This resarch was supported in part by Contract AT-(11-1) 1301 from the United States Atomic Energy Commission and Grant No. GB-13654 from the National Science Foundation.
Rights and permissions
About this article
Cite this article
Mills, D.I., Ellingboe, A.H. Somatic recombination in the common-AB diploid of Schizophyllum commune . Molec. Gen. Genet. 110, 67–76 (1971). https://doi.org/10.1007/BF00276048
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00276048