Summary
The mutation cIIts612 was found to map outside the immunity region of phage λimm21 hybrid. As expected of a cII mutation, λcIIts612 is unable to stimulate either cI repressor or Int synthesis during the establishment of lysogeny. These results indicate that part of the cII gene of λ is homologous to that of λimm21 phage.
This is a preview of subscription content, log in via an institution to check access.
References
Arber, W., Rifat, A., Wauters-Willems, D., Kühnlein, U.: Host specificity of DNA produced by Escherichia coli XVI. Phage λ DNA carries a single site of affinity for A-specific restriction and modification. Molec. gen. Genet. 115, 195–207 (1972)
Belfort, M., Noff, D., Oppenheim, A.B.: Isolation characterization and deletion mapping of amber mutations in the cII gene of bacteriophage lambda. Cirology 63, 147–159 (1975)
Brachet, P., Thomas, R.: Mapping and functional analysis of y and cII mutants. Mutation Res. 7, 257–260 (1969)
Davidson, N., Szybalski, W.: Physical and chemical characteristics of lambda DNA. In: The bacteriophage lambda (Hershey, A.D., ed.). Cold Spr. Harb. Laboratory 45–82 (1971)
Echols, H., Green, L.: Establishment and maintenance of repression by bacteriophage lambda: The role of the cI, cII and cIII proteins. Proc. nat. Acad. Sci. (Wash.) 68, 2190–2194 (1971)
Fleming, H., Haselkorn, R.: The program of protein synthesis during heterocyst differentiation in nitrogen-fixing blue-green algae. Cell 3, 159–170 (1974)
Hayes, S., Szybalski, W.: Control of short leftward transcripts from the immunity and ori regions in induced coliphage lambda. Molec. gen. Genet. 126, 275–290 (1973)
Herkowitz, I.: Control of gene expression in bacteriophage lambda. Ann. Rev. Genet. 7, 289–324 (1973)
Katzir, N., Oppenheim, A., Belfort, M., Oppenheim, A.B.: Activation of the lambda int gene by the cII and cIII gene products. Submitted for publication (1976)
Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.) 227, 680–685 (1970)
Lengeyl, J.A., Goldstein, R.N., Marsh, M., Sunshine, M., Calendar, R.: Bacteriophage P2 head morphogenesis: cleavage of the major capsid protein. Virology 53, 1–23 (1973)
Oppenheim, A.B.: Internal inversion used to study regulation of the int gene in bacteriophage λ. Nature (Lond.) 261, 615–616 (1976)
Oppenheim, A.B., Noff, D.: Deletion mapping of trans dominant mutations in the λ repressor gene. Virology 64, 553–556 (1975)
Reichardt, L., Kaiser, A.D.: Control of λ repressor synthesis. Proc. nat. Acad. Sci. (Wash.) 68, 2185–2189 (1971)
Reichardt, L.F.: Control of bacteriophage lambda repressor synthesis after phage infection: The role of the N, cII, cIII and cro products. J. molec. Biol. 93, 267–288 (1975)
Simon, M.N., Davis, R.W., Davidson, N.: Heteroduplexes of DNA molecules of lambdoid phages: Physical mapping of their base sequence relationships by electron microscopy. In: The bacteriophage lambda (Hershey, A.D., ed.). Cold Spr. Harb. Laboratory 315–328 (1971)
Zissler, J., Signer, E., Schaefer, F.: The role of recombination in growth of bacteriophage lambda. I. The gamma gene. In: The bacteriophage lambda (Hershey, A.D., ed.). Cold Spr. Harb. Laboratory 455–468 (1971)
Author information
Authors and Affiliations
Additional information
Communicated by G. Bertrani
Rights and permissions
About this article
Cite this article
Oppenheim, A.B., Kapeller, I. Analysis of a temperature sensitive mutation in gene cII of bacteriophage lambda. Molec. Gen. Genet. 149, 121–123 (1976). https://doi.org/10.1007/BF00275967
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00275967