Abstract
Radioiodinated antitumor (Ab-gamma globulins), non-tumor-specific Ab, and R131ISA were used for imaging radiation-induced intestinal tumors in rats. Each agent detected tumors larger than 2 g, but labeled Ab were most efficient in detecting smaller tumors. Tissue distribution studies showed that while ‘purified’ Ab localized specifically in tumors, ‘unpurified’ Ab concentrated in the tumor by a mechanism not considered immunological. Localization was variable and the concentration of antitumor Ab reached useful levels only in a small number of cases. The use of high specific activity purified Ab unexpectedly decreased the concentrations of label observed in the tumors when compared with the use of the same activity of low specific activity purified Ab. These results indicated the presence of circulating tumor antigens which were capable of binding the injected Ab. Subsequently, these findings have been substantiated. Thus the animal-to-animal variability could be explained on the basis of differing degrees of interaction of injected Ab with circulating tumor antigens. The usefulness of labeled purified or monospecific antitumor antibodies for tumor imaging and therapy would thus be influenced by the extent of such interactions.
Similar content being viewed by others
References
Bale WF, Spar IL, Goodland RL, Wolfe DE (1955) In vivo and in vitro studies of labeled antibodies against rat kidney and Walker carcinoma. Proc Soc Exp Biol Med 89:564–568
Ballou B, Levine G, Hakala TR, Solter D (1979) Tumor location detected with radioactivity-labeled monoclonal antibody and external scintigraphy. Science 206:844–846
Belitsky P, Ghose T, Acquino J, Norvell ST, Blair AH (1978) Radionuclide imaging of primary renal-cell carcinoma by I-131-labeled antitumor antibody. J Nucl Med 19:427–430
Cheng HF, Stevens RH, Cole DA (1980) Common circulating oncofetal protein in X-ray and chemically induced rat gastrointestinal cancer. Radiat Res 83:456
Coop KL, Sharp JG, Osborne JW, Zimmerman GR (1974) An animal model for the study of small bowel tumors. Cancer Res 34:1487–1494
Day ED, Mahaley MS, Woodhall B, Pircher F (1964) Localization of purified I125-anti-tumor radioantibodies in human brain tumors. J Nucl Med 5:357–358
Day ED, Lassiter S, Mahaley MS (1965) The localization of radioantibodies in human brain tumors. III. Radioiodination of prepurified localizing antibody. J Nucl Med 6:38–52
Day ED (1965) In: Jugelmass N (ed) The immunochemistry of cancer. Charles C Thomas, Springfield, Illinois, pp 1–170
Godenberg DM, DeLand F, Kim E, Bennetts S, Primus FJ, vanNagel JR, Estes N, DeSimone P, Rayburn P (1978) Use of radiolabeled antibodies to carcinoembryonic antigen for the detection and localization of diverse cancers by external photoscanning. New Engl J Med 298:1384–1388
Grabar P (1964) The immuno-electrophoretic method of analysis. In: Graba P, Burtin P (eds) Immuno-electrophoretic method of analysis. Elsevier, New York, Ch 1
Greenwood FC, Hunter WM, Glover JS (1963) The preparation of 131I-labeled human growth hormone of high specific radioactivity. Biochem J 89:114–123
Hunter R (1970) Standardization of the chloramine-T method of protein iodination. Proc Soc Exp Biol Med 133:989–992
Izzo MJ, Buchsbaum DJ, Bale WF (1972) Localization of an 125I-labeled rat transplantation antibody in tumors carrying the corresponding antigen. Proc Soc Exp Biol Med 139:1185–1188
Jagendorf AT, Patchornik A, Sela M (1965) Use of antibody bound to modified cellulose as an immunospecific adsorbent of antigens. Biochem Biophys Acta 78:516–527
Korngold L (1967) Serological techniques for the analyses of tumor antigens. In: Busch H (ed) Methods in cancer research, Vol 2. Academic Press, New York, p 45
Lawson AJ, Cheng HF, Osborne JW, Stevens RH (1979) Serum levels of carcinoembryonic antigen-reactive substance (CEA-rs) in rats bearing an X-irradiation induced small bowel adenocarcinoma. In: Lehmann FG (ed) Carcino-embryonic proteins, Vol II. Elsevier/North Holland Biomedical Press, Amsterdam, p 47–52
Osborne JW, Zimmerman GR, Prasad KN (1966) Production in rats of a lesion resembling intestinal adenocarcinoma. In: Third International Congress of Radiation Research Book of Abstracts, Cortina, Italy, p 171
Ouchterlony O (1949) Antigen-antibody reactions in gels. Acta Pathol Microbiol Scand 26:507–515
Ouchterlony O (1953) Antigen-antibody reactions in gels. IV. Types of reactions in coordinated systems of diffusion. Acta Pathol Microbiol Scand 32:231–240
Pressman D (1957) Radiolabeled antibodies. Ann NY Acad Sci 69:644–650
Quinones J, Mizejewski G, Beierwaltes WH (1971) Choriocarcinoma scanning using radiolabelled antibody to chorionic gonadotrophin. J Nucl Med 12:69–75
Reif AE (1971) Studies on the localization of radiolabeled antibodies to a mouse myeloma protein. Cancer 27:1433–1439
Reyes EL, Talley RW (1970) Primary malignant tumors of the small intestine. Am J Gastroenterol 54:30–43
Robbins JB, Haimovich J, Sela M (1967) Purification of antibodies with immunoadsorbents prepared using bromoacetyl cellulose. Immunochemistry 4:11–22
Scheidegger JJ (1955) Une Micro-methode de l-immunoelectrophorese. Int Arch Allerg 7:103–110
Sharp JG, Coop KL, Osborne JW (1973) Hematopoiesis in rats with radiation-induced small bowel tumors. Radiat Res 55:588
Sonoda S, Schlamowitz M (1970) Studies of 125I trace labeling of immunoglobulin G by chloramine-T. Immunochemistry 7:885–898
Stevens RH, Englund CW, Osborne JW, Cheng HF, Richerson HB (1975) Oncofetal protein accompanying irradiation induced small bowel adenocarcinoma in the rat. J Natl Cancer Inst 55:1011–1013
Stevens RH, Englund CW, Osborne JW, Cheng HF, Hoffman KL (1976) Identification and characterization of a circulating tumor-associated oncofetal protein from a radiation induced adenocarcinoma of the rat small bowel. Cancer Res 36:3260–3264
Witz I, Klein G, Pressman D (1969) Moloney lymphoma antibodies from mice; localization in spleens of Moloney lymphoma bearing mice. Proc Soc Exp Biol Med 130:1102–1105
Yagi Y, Pressman D (1964) Pair-labeled antitumor antibodies. J Nucl Med 5:310–311
Author information
Authors and Affiliations
Additional information
This work was presented in part at the Twentieth Annual Meeting of the Radiation Research Society, Portland, Oregon, May, 1972.
Rights and permissions
About this article
Cite this article
Sharp, J.G., Osborne, J.W., Cheng, H.F. et al. Scintigraphy and distribution of labeled antibodies in rats with tumors. Eur J Nucl Med 7, 28–34 (1982). https://doi.org/10.1007/BF00275241
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00275241