Abstract
Wyman's equation for facilitated diffusion of a ligand through a solution slab containing a carrier is recast and solved by means of a regular perturbation expansion in the parameter representing the driving force for facilitation. This new solution is complementary to the previously exploited singular perturbation solution due to Murray and represents facilitation in the low facilitation parameter regime. The most significant physical realization of this regime occurs when there is a large affinity between ligand and carrier, as in the carbon monoxide-hemoglobin system.
The validity domains of the regular perturbation solution and the singular perturbation solution of Mitchell and Murray and Rubinow and Dembo are delineated. The equation for facilitated diffusion is solved numerically for parameter values appropriate to the oxygen-myoglobin experiments of Wittenberg, and to the carbon monoxide-hemoglobin experiments of Mochizuki and Forster, and Wittenberg. This solution provides a norm for comparison of the utility of the perturbation solutions.
We show how the theory explains the apparent contradiction between the positive observations of Mochizuki and Forster and the negative observations of Wittenberg on facilitation of carbon monoxide transport through a slab of hemoglobin solution.
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References
Abromowitz, M., Stegun, I.A.: Handbook of mathematical functions. Washington, DC: National Bureau of Standards 1964
Basset, R. J., Schultz, J. S.: Nonequilibrium facilitated diffusion of oxygen through membranes of aqueous cobaltohistidine. Biochem. Biophys. Acta 211, 194–215 (1970)
Crank, J.: The mathematics of diffusion, Vol. VIII. Oxford: Clarendon Press 1956
Gibson, Q. H.: The kinetics of reactions between haemoglobin and gases. In: (Butler, J. A. V., Katz, B., eds.) Progress in biophysics and biophysical chemistry, pp. 1–53. London: Pergamon 1959
Goddard, J. D., Schultz, J. S., Basset, R. J.: On membrane diffusion with near-equilibrium reaction. Chem. Eng. Sci. 25, 665–683 (1970)
Goddard, J. D., Schultz, J. S., Suchdeo, S. R.: Facilitated transport via carrier-mediated diffusion in membranes: Part II. Mathematical aspects and analyses. AICHE Journal 20, 625–645 (1974)
Hoofd, L., Kreuzer, F.: A new mathematical approach for solving carrier-facilitated steady-state diffusion problems. J. Math. Biol. 8, 1–13 (1979)
Kreuzer, F., Hoofd, L.: Facilitated diffusion of CO and oxygen in the presence of hemoglobin or myoglobin. In: Oxygen transport to tissue-II (Adv. Exper. Med. Biol., Vol. 75) pp. 207–215. New York-London: Plenum Press 1976
Kutchai, H., Jacquez, J. A., Mather, F. J.: Nonequilibrium facilitated oxygen transport in hemoglobin solution. Biophys. J. 10, 38–54 (1970)
Laforce, R. C.: Steady-state diffusion in the carbon monoxide + oxygen + haemoglobin system. Trans. Faraday Soc. 62, 1458–1468 (1966)
Linke, W. F.: Solubilities. Princeton: D. Van Nostrand 1958
Mitchell, P. J., Murray, J. D.: Facilitated diffusion: The problem of boundary conditions. Biophysik. 9, 177–190 (1973)
Mochizuki, M., Forster, R. E.: Diffusion of carbon monoxide through thin layers of hemoglobin solution. Science 138, 897–898 (1962)
Murray, J. D.: On the molecular mechanism of facilitated oxygen diffusion of haemoglobin and myoglobin. Proc. R. Soc. London Ser. B. 178, 95–110 (1971)
Murray, J. D., Wyman, J.: Facilitated diffusion. The case of carbon monoxide. J. Biol. Chem. 246, 5903–5906 (1971)
Nedelman, J.: Ithaca, NY: Ph.D. Thesis. Cornell University 1981
Riveros-Moreno, V., Wittenberg, J. B.: The self-diffusion coefficients of myoglobin and hemoglobin in concentrated solutions. J. Biol. Chem. 247, 895–901 (1972)
Roughton, F. J. W.: In: Handbook of respiratory physiology, p. 59. Randolph Field, TX: USAF School of Aviation Medicine 1954
Rubinow, S. I., Dembo, M.: The facilitated diffusion of oxygen by hemoglobin and myoglobin. Biophys. J. 18, 29–42 (1977)
Smith, K. A., Meldon, J. H., Colton, C. K.: An analysis of carrier-facilitated transport. AICHE J. 19, 102–111 (1973)
Snell, F. M.: Facilitated transport of oxygen through solutions of hemoglobin. J. Theor. Biol. 8, 469–479 (1965)
Widdas, W. F.: Inability of diffusion to account for placental glucose transfer in the sheep and consideration of the kinetics of a possible carrier transfer. J. Physiol. 118, 23–39 (1952)
Wittenberg, J. B.: The molecular mechanism of hemoglobin-facilitated oxygen diffusion. J. Biol. Chem. 241, 104–114 (1966)
Wittenberg, J. B.: Myoglobin-facilitated oxygen diffusion: Role of myoglobin in oxygen entry into muscle. Physiol. Rev. 50, 559–636 (1970)
Wyman, J.: Facilitated diffusion and the possible role of myoglobin as a transport mechanism. J. Biol. Chem. 241, 115–121 (1966)
Yung, D., Probstein, R. F.: Similarity considerations in facilitated transport. J. Phys. Chem. 77, 2201–2205 (1973)
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Supported in part by the National Science Foundation under Grant No. PCM77-03344
In partial fulfillment of the Ph.D. degree at Cornell University
Professor S. I. Rubinow has passed away on February 22, 1981
Computations were performed at the Courant Mathematics and Computing Laboratory, supported by DOE under contract #EY-76-C-02-3077.
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Nedelman, J., Rubinow, S.I. Facilitated diffusion of oxygen and carbon monoxide in the large affinity regime. J. Math. Biol. 12, 73–90 (1982). https://doi.org/10.1007/BF00275204
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DOI: https://doi.org/10.1007/BF00275204