Summary
The SR180 cell line has been isolated in a callus culture derived from a haploid Nicotiana sylvestris (n = X = 12) plant by its ability to proliferate on a selective medium containing 2,000 μg/ml streptomycin sulphate. From the cell line diploid plants have been regenerated. The SR180 selfs are resistant to streptomycin. Streptomycin sensitivity in F1, and a 3∶1 (sensitive to resistant) segregation in F2 indicate that resistance in the SR180 mutant is the result of a recessive Mendelian mutation.
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Communicated by G. Melchers
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Maliga, P. Streptomycin resistance is inherited as a recessive Mendelian trait in a Nicotiana sylvestris line. Theoret. Appl. Genetics 60, 1–3 (1981). https://doi.org/10.1007/BF00275170
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DOI: https://doi.org/10.1007/BF00275170