Summary
To evaluate the effect of sulphonylurea on in vivo tissue uptake of glucose, the arterial injection tissue-sampling technique of Oldendorf [9] was used to measure the glucose uptake of brain; liver; subcutaneous fat; and of three skeletal muscles, the masseter, femoris and soleus. Rats gavaged with glipizide 5 mg/kg daily for 5 days were compared to vehicletreated rats. The serum glucose levels at the time of the experiment were identical in the two groups (10.36±0.42 mmol/l vs 10.38±0.36 mmol/l). Glipizide treatment did not result in an increase in glucose uptake by the various tissues studied. It is concluded that, under physiological conditions in non-fasted rats, sulphonylureas do not significantly alter tissue uptake of glucose.
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Pfeiffer EF, Pfeiffer M, Ditschuneit H, Ahn CS (1959) Clinical and experimental studies of insulin secretion following tolbutamide and metehexamide administration. Ann NY Acad Sci 82: 479–495
Yalow RS, Black H, Villazon M, Berson SA (1960) Comparison of plasma insulin Levels following administration of tolbutamide and glucose. Diabetes 9: 356–362
Levey GS (1977) The effects of sulfonylureas on peripheral metabolic process. Fed Proc 36: 2720–2723
Duckworth WC, Solomon SS, Kitabchi AE (1972) Effect of chronic sulfonylurea therapy on plasma insulin and proinsulin levels. J Clin Endocrinol Metab 35: 585–591
Feinglos MN, Lebovitz HE (1978) Sulphonylureas increase the number of insulin receptors. Nature 276: 184–185
Jacobs DB, Jung CY (1985) Sulfonylurea potentiates insulin-induced recruitment of glucose transport carrier in rat adipocytes. J Biol Chem 260: 2593–2596
Greenfield MS, Doberne L, Rosenthal M, Shulz B, Widstrom A, Reaven GN (1982) Effect of sulfonylurea treatment on in vivo insulin secretion and action in patients with non-insulin dependent diabetes mellitus. Diabetes 31: 307–312
Verdonk CA, Rizza R, Gerich JE (1981) Effects of plasma glucose on glucose utilization and glucose clearance in normal man. Diabetes 30: 535–537
Oldendorf WH (1970) Measurement of brain uptake of radiolabelled substances using a tretiated water internal standard. Brain Res 24: 372–376
Bonen A, Tan MH, Watson-Wright WM (1981) Insulin binding and glucose uptake differences in rodent skeletal muscles. Diabetes 30: 702–704
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Mooradian, A.D. The effect of sulphonylurea on the in vivo tissue uptake of glucose in normal rats. Diabetologia 30, 120–121 (1987). https://doi.org/10.1007/BF00274583
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DOI: https://doi.org/10.1007/BF00274583