Summary
Variation in polypeptide synthesis was examined in developing maize embryos of two inbred and two hybrid genotypes. Multivariate analyses were used to evaluate the variation among two-dimensional, electrophoretic separations of polypeptides. Several features of the data set were revealed. Similar developmental patterns were exhibited by all genotypes and no evidence was obtained for differential rates of development for inbreds and hybrids. The differential synthesis of two subsets of polypeptides during embryo development was observed. The multivariate methods employed in this study were a valuable aid in interpreting the results from a large and complex data set.
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References
Aliaga-Morell JR, Culianez-Macia FA, Clémente-Marin G, Primo-Yufera E (1987) Differentiation of rice varieties by electrophoresis of embryo protein. Theor Appl Genet 74:224–232
Anderson NL, Hofmann J-P, Gemmell A, Taylor J (1984) Global approaches to quantitative analysis of gene expression patterns observed by use of two-dimensional gel electrophoresis. Clin Chem 30:2031–2036
Bahrman N, Thiellement H (1987) Parental genome expression in synthetic wheats (Triticum turgidum sp. x T. tauschii sp.) revealed by two-dimensional electrophoresis of seedling proteins. Theor Appl Genet 74:218–223
Baszczynski CL, Walden DB, Atkinson BG (1983) Regulation of gene expression in corn (Zea mays L.) by heat shock. II. In vitro analysis of RNAs from heat-shocked seedlings. Can J Biochem Cell Biol 61:395–403
Bonner WM, Laskey RA (1974) A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem 43:83–88
Bretting PK, Goodman MM, Stuber CW (1987) Karyological and isozyme variation in West Indian and allied American mainland races of maize. Am J Bot 74:1601–1613
Damerval C, Herbert Y, de Vienne D (1987) Is the polymorphism of protein amounts related to phenotypic variability? A comparison of two-dimensional electrophoresis data with morphological traits in maize. Theor Appl Genet 74:194–202
Dunbar BD, Bundman DS, Dunbar BS (1985) Identification of cultivar-specific proteins of winter wheat (T. aestivum L.) by high resolution two-dimensional polyacrylamide gel electrophoresis and color-based silver stain. Electrophoresis 6:39–43
Dure L (1985) Embryogenesis and gene expression during seed formation. In: Miflin BJ (ed) Oxford surveys of plant molecular and cell biology, vol 2. Oxford University Press, New York, pp 67–69
Dure L, Greenway SC, Galau GA (1981) Developmental biochemistry of cottonseed embryogenesis and germination: changing messenger ribonucleic acid populations as shown by in vitro and in vivo protein synthesis. Biochemistry 20:4162–4168
Feoli E, Orlóci L (1979) Analysis of concentration and detection of underlying factors in structured tables. Vegetatio 1:49–54
Feoli E, Orlóci L (1985) Species dispersion profiles of anthropogenic grasslands in the Italian Eastern Pre-Alps. Vegetatio 60:113–118
Fewster PH, Walden DB (1987) Multivariate analysis of data from two-dimensional electrophoretic separation of macromolecules. Comput Biol Med 17:29–35
Gower JC (1966) Some distance properties of latent root and vector methods in multivariate analysis. Biometrika 53:315–328
Hughes WG, Baszczynski CL, Ketola-Pirie C (1981) Improved conditions for the extraction of maize polypeptides. Maize Genet Coop Newslett 55:116–117
Kreis M, Shewry PR, Forde BG, Forde J, Miflin BJ (1985) Structure and evolution of seed storage proteins and their genes with particular reference to those of wheat, barley and rye. In: Miflin BJ (ed) Oxford surveys of plant molecular and cell biology, vol 2. Oxford University Press, New York, pp 253–317
Kriz AL (1982) In vivo labeling of embryo and endosperm proteins in intact kernels with 35-S methionine. Maize Genet Coop Newslett 56:14–15
Laskey RA, Mills AD (1975) Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem 56:335–341
Mans RJ, Novelli DG (1960) A convenient, rapid and sensitive method for measuring the incorporation of radioactive amino acids into protein. Biochem Biophys Res Commun 3:540–548
Misra S, Bewley JD (1985) Reprogramming of protein synthesis from a developmental to a germinative mode induced by desiccation of the axes of Phaseolus vulgaris. Plant Physiol 78:876–882
O'Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021
Orlóci L (1981) Probing time series vegetation data for evidence of succession. Vegetatio 46:31–35
Sanchez-Martinez D, Puigdomenech P, Pages M (1986) Regulation of gene expression in developing Zea mays embryos. Plant Physiol 82:543–549
Sass JE (1976) Morphology. In: Sprague GF (ed) Corn and corn improvement. American Society of Agronomy, Madison/WI, pp 89–110
Sawney VK, Chen K, Sussex IM (1985) Soluble proteins of the mature floral organs of tomato (Lycopersicon esculentum Mill). J Plant Physiol 121:265–271
Soave C, Salamini F (1984) The role of structural and regulatory genes in the development of maize endosperm. Dev Genet 5:1–25
Smith JSC (1984) Genetic variability within US hybrid maize: Multivariate analysis of isozyme data. Crop Sci 24:1041–1046
Smith JSC, Smith OS (1987) Associations among inbred lines of maize using electrophoretic, chromatographic and pedigree data. Theor Appl Genet 73:654–664
Tellenaar M, Muldoon JF, Daynard TB (1984) Differences in rates of leaf appearance among maize hybrids and phases of development. Can J Plant Sci 64:759–763
Zivy M, Thiellement H, Vienne D de, Hofmann J-P (1983) Study on nuclear and cytoplasmic genome expression in wheat by two-dimensional gel electrophoresis. 1. First results on 18 alloplasmic lines. Theor Appl Genet 66:1–7
Zivy M, Thiellement H, Vienne D de, Hofmann J-P (1984) Study on nuclear and cytoplasmic gene expression in wheat by two-dimensional gel electrophoresis. 2. Genetic differences between two lines and two groups of cytoplasms at five developmental stages or organs. Theor Appl Genet 68:335–345
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Communicated by P. L. Pfahler
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Boothe, J.G., Walden, D.B. Multivariate analyses of polypeptide synthesis in developing maize embryos. Theoret. Appl. Genetics 77, 495–500 (1989). https://doi.org/10.1007/BF00274269
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DOI: https://doi.org/10.1007/BF00274269