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Soluble proteins and isoenzymes from seeds of diverse male steriles of sorghum, (Sorghum bicolor (L.) Moench)

Summary

A comparison of soluble protein, esterase, GDH and ADH isoenzyme patterns in seeds of different steriles, maintainers and restorer lines exhibited similarities as well as differences. Soluble protein patterns from sterile and maintainer lines differed both qualitatively and quantitatively. Based on the esterase patterns, male steriles with different cytoplasms could be separated into three groups (i) Ck 60A and B; Nagpur A and B, (ii) M 35-1A and 1 B, M 31-2A and 2B, (iii) G1A and B, VZM2A and 2B. Each group could further be differentiated on the basis of minor differences in esterase isoenzyme patterns within each group. ADH and GDH patterns in general were similar in both sterile and maintainer lines.

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Abbreviations

ADH:

Alcohol dehydrogenase

GDH:

Glutamate dehydrogenase

NAD:

Nicotinamide adenine dinucleotide

Literature

  • Alam, S.; Sandal, P.C. (1969): Electrophoretic analysis of anther protein from male fertile and male sterile Sudangrass, Sorghum vulgare var. ‘Sudanense (Piper)’. Crop Sci. 9, 157–159

    Google Scholar 

  • Davis, B.J. (1964): Disc electrophoresis. 2. Method and application to human serum proteins. Ann. N.Y. Acad. Sci 20, 121–404

    Google Scholar 

  • Ornstein, L. (1964): Disc electrophoresis. 1. Background and theory. Ann. N.Y. Acad. Sci. 121, 321–340

    Google Scholar 

  • Payne, R.C., Koszykovski, T.T. (1978): Esterase isoenzyme differences in seed extracts among soybean cultivars. Crop Sci. 18, 557–559

    Google Scholar 

  • Siddiq, E.A., Nerkar, Y.S., Mehta, S.L. (1972): Intra and inter subspecific variation in soluble proteins of Oryza sativa L. Theor. Appl. Genet. 42, 351–356

    Google Scholar 

  • Schwartz, D. (1966): The genetic control of Alcohol dehydrogenase in maize: gene duplication and repression. Proc. Natl. Acad. Sci. USA 56, 1431–1436

    Google Scholar 

  • Yanofsky, C.; Lawrence, P. (1960): Gene action. Ann. Rev. Microbiol. 14, 311–340

    Google Scholar 

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Communicated by B. R. Murty

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Tripathi, D.P., Mehta, S.L. & Rao, N.G.P. Soluble proteins and isoenzymes from seeds of diverse male steriles of sorghum, (Sorghum bicolor (L.) Moench). Theoret. Appl. Genetics 64, 325–331 (1983). https://doi.org/10.1007/BF00274173

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  • DOI: https://doi.org/10.1007/BF00274173

Key words

  • Isoenzyme
  • Male sterile (A)
  • Maintainer (B)
  • Restorer (R)
  • Apomict
  • Sorghum bicolor (L.) Moench
  • Gel electrophoresis