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Virulence of Agrobacterium tumefaciens strains with Brassica napus and Brassica juncea

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Summary

Brassica napus and Brassica juncea were infected with a number of Agrobacterium tumefaciens strains. Tumourigenesis was very rapid and extremely efficient on B. juncea with all but one of the strains. Tumourigenesis on B. napus varied widely. It was very efficient with the nopaline strains, was reduced with the succinamopine strain A281 and was very weak with the octopine strains. The latter observation was confirmed with six different B. napus rapeseed cultivars. The selectivity was due to differences in the virulence of Ti plasmids with B. napus, rather than the tumourigenicity of the T-DNA or virulence of the chromosomal genes associated with the strains. An exception was strain LBA4404. The virulence of the octopine strains was increased by coinfection with more virulent disarmed strains and by induction with acetosyringone.

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Author notes

  1. Pierre J. Charest

    Present address: Petawawa National Forestry Institute, Forestry Canada, K0J 1J0, Chalk River, Ontario, Canada

Authors and Affiliations

  1. Department of Biology, Carleton University, K1S 5B6, Ottawa, Ontario, Canada

    Pierre J. Charest & V. N. Iyer

  2. Plant Research Centre, Agriculture Canada, K1A 0C6, Ottawa, Ontario, Canada

    Brian L. Miki

Authors
  1. Pierre J. Charest
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  2. V. N. Iyer
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  3. Brian L. Miki
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Communicated by F. Constabel

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Charest, P.J., Iyer, V.N. & Miki, B.L. Virulence of Agrobacterium tumefaciens strains with Brassica napus and Brassica juncea . Plant Cell Reports 8, 303–306 (1989). https://doi.org/10.1007/BF00274136

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  • DOI: https://doi.org/10.1007/BF00274136

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