Abstract
Somatic embryogenesis and plant regeneration of Canada wildrye (Elymus canadensis L.) from tissue culture was investigated by culturing immature embryos and inflorescences on MS medium containing 2 mg/l 2,4-D. The optimum size of explants for maximum embryogenic callus formation was 1.0 to 1.5 mm for embryos and 4 to 6 cm for inflorescences. Plant regeneration from the subcultured embryogenic callus was attempted monthly using hormone-free MS medium or MS medium with 0.5 mg/1 2,4-D and 0.3 mg/l GA3. Three hundred and fifty seven plantlets were regenerated from the callus cultures of both explant sources during a six month period. Ten chlorophyll deficient plants accounting for 2.8% of the total regenerants were observed. One plant with white striped leaves survived and was found to be an octoploid.
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Abbreviations
- GA3:
-
gibberellic acid
- MS:
-
Murashige and Skoog (1962)
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
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Park, C.H., Walton, P.D. Embryogenesis and plant regeneration from tissue culture of Canada wildrye, Elymus canadensis L.. Plant Cell Reports 8, 289–291 (1989). https://doi.org/10.1007/BF00274132
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DOI: https://doi.org/10.1007/BF00274132