Summary
An enrichment procedure that exploits the difference in heat-sensitivity between exponentially growing and stationary phase cells has been developed for the isolation of yeast mutants. Enrichments of up to 12-fold for temperature-sensitive lethal mutants and of up to 15-fold for auxotrophs have been obtained with single cycles of selection. Still higher enrichments (to frequencies of greater than 90% and 80% for temperature-sensitive lethals and auxotrophs, respectively) have been obtained with multiple cycles of selection. The method requires no special parent strain, and seems adaptable to the selection of a wide variety of types of mutants.
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Communicated by Ch. Auerbach
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Walton, E.F., Carter, B.L.A. & Pringle, J.R. An enrichment method for temperature-sensitive and auxotrophic mutants of yeast. Molec. Gen. Genet. 171, 111–114 (1979). https://doi.org/10.1007/BF00274022
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DOI: https://doi.org/10.1007/BF00274022