Summary
Sheared DNA from RPI, an R plasmid from a strain of Pseudomonas aeruginosa, was used to transform other strains of P. aeruginosa and Escherichia coli. From transformed cells other plasmids like RPI were isolated. These deletion plasmids were conjugally transferrable and confer resistance mainly against carbenicillin and tetracycline.
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References
Chakrabarty, A.M., Mylroie, J.R., Friello, D.A., Vacca, J.G.: Transformation of Pseudomonas putida and Escherichia coli with plasmid-linked drug-resistance factor DNA. Proc. Nat. Acad. Sci. USA 72, 3647–3651 (1975)
Cohen, S.N., Chang, A.C.Y., Hsu, I.: Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc. Nat. Acad. Sci. USA 69, 2110–2114 (1972)
Goebel, W., Bonewald, R.: Class of small multicopy plasmids originating from the mutant antibiotic resistance factor RIdrd-19B2. J. Bact. 123, 658–665 (1975)
Grinsted, J., Saunders, J.R., Ingram, L.C., Sykes, R.B., Richmond, M.H.: Properties of an R factor from Pseudomonas aeruginosa. J. Bact. 110, 529–537 (1972)
Lowbury, E.J., Kidson, A., Lily, H.A., Ayliffe, G.A.J., Jones, R.J.: Sensitivity of Pseudomonas aeruginosa to antibiotics: emergence of strains resistance to carbenicillin. Lancet II, 448–452 (1969)
Roe, E., Jones, R.J., Lowbury, E.J.: Transfer of antibiotic resistance between Pseudomonas aeruginosa, Escherichia coli and other Gram-negative bacillus in burns. Lancet I, 149–152 (1972)
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Communicated by O. Siddiqi
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Haque, H. Transformation of Pseudomonas aeruginosa and Escherichia coli with resistance plasmid DNA: Formation of smaller conjugative plasmids from RPI. Molec. Gen. Genet. 171, 107–109 (1979). https://doi.org/10.1007/BF00274021
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DOI: https://doi.org/10.1007/BF00274021