Summary
A restriction endonuclease cleavage map of phage P2 was constructed. The enzymes used and, within parenthesis, the number of their cleavage sites on the P2 lg cc DNA molecule were: AvaI(3), BalI(1), BamI(3), BglII(3), HaeIII (more than 40; only three were mapped), HindIII(0), HpaI(10), KpnI(3), PstI(3), SalI(2) and SmaI(1). The EcoRI cleavage sites (3), as determined earlier, were used as reference points for this study. The DNAs of a variety of P2 mutants carrying chromosomal aberrations (del1, del2, del3, del6, vir22, vir37(2), vir79 and vir94) were also similarly examined.
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Communicated by W. Arber
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Westöö, A., Ljungquist, E. A restriction endonuclease cleavage map of bacteriophage P2. Molec. Gen. Genet. 171, 91–102 (1979). https://doi.org/10.1007/BF00274019
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DOI: https://doi.org/10.1007/BF00274019