Summary
Data obtained from simultaneous determinations of serum creatine-kinase levels and estimation of ultrasound attenuation values in muscles greatly improved the detection of obligate carriers of Duchenne muscular dystrophy than when only one of these methods was employed alone. Eleven carriers out of 19 had a high creatine-kinasemia level and nine carriers out of 19 had a high (abnormal) attenuation value. Because of the limited overlapping between the two parameters studied, we were able to recognize 17 obligate carriers out of the 19. This indicates that the parameters studied concern different features of the disease, and the practical and theoretical considerations are discussed. The techniques are discussed together with molecular genetic investigations.
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References
Adornato BT, Kagen LJ, Engel WE (1978) Myoglobinacmia in Duchenne muscular dystrophy patients and carriers, a new adjunct to carrier detection. Lancet II:499–501
Afifi AKR, Borgman RA, Zellweger H (1973) A possible role for electron miscroscopy in the detection of carriers of Duchenne type muscular dystrophy. J Neurol Neurosurg Psychiatry 36:643–650
Alberts MC, Samaha FJ (1974) Serum pyruvate kinase in muscle disease and carrier states. Neurology 5:462–464
Berger G, Perrin J (1980) Attenuation principles and measurements for tissue characterization. In: Thijssen JM (ed) Ultrasonic tissue characterization: clinical achievement and technological potentials. Staffleu's Scientific Publishing Company, Alphen a/d Riin, Holland, pp 177–185
Boulé M, Vanasse M, Brakier-Gingras L (1979) Decrease in the rate of protein synthesis by polysomes from cultured fibroblasts of patients and carriers with Duchenne muscular dystrophy. Can J Neurol Sci 6:355–357
Brown MD, Chattophaday SK, Patel AB (1967) Erythrocyte abnormality in human myopathy. Science 157:1577–1578
Burt D, Emery AEH (1979) Serum LDH.5 in carriers of Duchenne muscular dystrophy. Neurology 29:239–241
Comi G, Gomola M, Galardo G, Ronzoni MG, Piattoni F, Erenbourg L, Canal N (1982) Duchenne dystrophy carrier detection: electromyography and enzymes. Cardiomyology 1/2:173–180
Danieli GA, Angelini G (1976) Duchenne carrier detection. Lancet II:70–72
Dreyfus JC, Schapira F, Demos J, Rosa R, Schapira G (1966) The value of serum enzyme determinations in the identification of dystrophic carriers. Ann NY Acad Sci 138:304–314
Dubowitz V, Brooke MH (1973) Duchenne carrier dystrophy. Carrier detection. In: Walton JN (ed) Muscle biopsy a modern approach. (Major problems in neurology, vol 2) Saunders, London, p 179
Ebashi SY, Toyokura HM, Sugita H (1956) High creatine phosphokinase activity of sera of progressive muscular dystrophy patients. J Biochem (Tokyo) 46:103–110
Emery AEM, Skinner R, Molloway S (1979) A study of possible hetrogeneity in Duchenne muscular dystrophy. Clin Genet 15:444
Fink M, Hottier F, Cardoso JF (1983) Ultrasonic signal processing for in vivo attenuation measurements: short time Fourier analysis. Ultrasonic Imaging 5:117–135
Fischer S, Tortolero M, Piau JP, Delaunay J, Schapira G (1980) Phosphorylation and dephosphorylation reactions by erythrocyte plasma membrane enzymes. Biochim Biophys Acta 598:463–471
Heckmatt JZ, Dubowitz V (1983) Detecting the Duchenne carrier by ultrasound and computerised tomography. Lancet I:1364
Tonesescu V, Zellweger H, Burmeister L (1976) Detection of carriers and genetic counselling in Duchenne muscular dystrophy by ribosomal protein synthesis. Acta Neurol Scand 54:442–452
Lane RJM, Roses AD (1981) Variation of serum creatine kinase levels with age in normal females: implications for genetic counselling in Duchenne muscular dystrophy. Clin Chim Acta 75–86
Laugier P, Berger G, Fink M, Perrin J (1985) Specular reflector noise: effect and correction for in vitro attenuation estimation. Ultrasonic Imaging 7:277–292
Leterrier F, Lamas E, Daveloose D, Rochette J, Schapira G (1984) Spin labelling of the erythrocyte membrane in mothers and sisters of patients suffering from Duchenne muscular dystrophy. Clin Chim Acta 143:99–108
Lyon MF (1971) Possible mechanism of X chromosomal inactivation. Nature 232:229–232
Monaco PA, Bertelson CJ, Middlesworth W, Colletti CA, Aldridge J, Fischbeck KH, Bartlett R, Pericak-Vance MA, Roses AD, Kunkel LM (1985) Detection of deletions spanning the Duchenne muscular dystrophy locus using a tightly linked DNA segment. Nature 316:842–845
Murray JM, Davies KE, Harper PS, Williamson R (1982) Linkage relationship of a cloned DNA sequence on the short arm of the X chromosome to Duchenne muscular dystrophy. Nature 300:69–71
Oepkes CT, Van Weerden (1979) Relaxation time of the adductor pollicis muscle in Duchenne muscular dystrophy carriers: a new method fordetecting carriers. Neurology 29:523–524
Percy ME, Andrews DF, Thompson MW (1982) Serum creatine kinase in the detection of Duchenne muscular dystrophy: effects on season and multiple testing. Muscle Nerve 5:58–64
Roses AD, Herbstreith MA, Appel SM (1975) Membrane protein kinase alteration in Duchenne muscular dystrophy. Nature 254:350–351
Rott HD, Mulz D (1982) Muskeldystrophie Duchenne: Konduktorinnenerfassung mit Ultraschall. Dtsch Med Wochenschr 107:1678–1681
Rott HD, Santellani M, Rödl W, Nebel G (1983) Duchenne muscular dystrophy: carrier detection by ultrasound and computerised tomography. Lancet I:1199–1200
Schapira F, Dreyfus JC, Schapira G, Demos J (1960) Étude de l'aldolase et de la créatine kinase du sérum chez les mères de myopathes. Rev Fr Études Clin Biol 5:990–994
Schapira G, Dreyfus JC, Schapira F (1953) L'élévation du taux de l'aldolase sérique, test biochimique des myopathies. Sem Hôp Paris 29:1917–1921
Shumate JB, Brooke MH, Carroll MD, Davis SE (1979) Increased serum creatine kinase after exercise. Neurology 29:902–904
Simpson J, Zellweger M, Burmeister F, Christee R, Nielson KM (1974) Effect of oral contraceptive pills on the level of creatine phosphokinase with regard to carrier detection in Duchenne muscular dystrophy. Clin Chim Acta 52:219–223
Smith I, Elton RA, Thompson WMS (1979) Carrier detection in X-linked (Duchenne) muscular dystrophy: serum creatine phosphokinase values in prementarchal, menstruating, post-menopausal and pregnant normal women. Clin Chim Acta 98:207–216
Steinbicker V, Van Rohden L, Krebs P, Sziber R (1984) Duchenne muscular dystrophy: carrier detection by ultrasound. Lancet I:1463; II:1054
Steinbicker V, Von Rohden L, Gelleuch L, Szibor R (1985) Duchenne carrier diagnosis by use of ultrasonography and computed tomography. Clin Genet 28:468
Stern LM, Cauchy DJ, Clark MS, Penet LV, Bolok DW (1985) Carrier detection in Duchenne muscular dystrophy using computed tomography. Clin Genet 27:392–397
The Committee on Enzymes of the Scandinavian Society (1976) Scand J Clin Lab Invest 36:711–723
Verrill HL, Pickard NA, Gruemer HD (1977) Diminished cap formation in lymphocytes from patients and carriers of Duchenne muscular dystrophy. Clin Chem 23:2341–2344
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Schapira, G., Laugier, P., Rochette, J. et al. Detection of duchenne muscular dystrophy carriers: quantitative echography and creatine kinasemia. Hum Genet 75, 19–23 (1987). https://doi.org/10.1007/BF00273832
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DOI: https://doi.org/10.1007/BF00273832