Summary
Incorporation of (methyl-14C)choline into phosphatidylcholine and the release of prelabelled phosphatidylcholine was investigated in vitro using lung slices from pregnant rats and their offspring near term. Tissue from normal, diabetic and insulin-treated diabetic pregnant rats and their offspring was utilized to assess the effects of maternal diabetes on fetal lung maturation. The results show that the synthesis of phosphatidylcholine in fetal/newborn lungs through the cytidine 5′-diphosphocholine pathway was not affected by maternal diabetes. However, secretion of phosphatidylcholine from the lungs of fetuses of diabetic mothers was very much suppressed one day after parturition. Insulin treatment of the diabetic pregnant rats restored secretion of phosphatidylcholine from the fetal/newborn lungs to control values. These re sults suggest that an impaired secretion of phosphatidylcholine from the lungs of fetuses of diabetic mothers may be partly responsible for the higher incidence of respiratory distress syndrome among children of diabetic mothers. The results also revealed some correlation between the secretion of phosphatidylcholine from maternal lungs, maternal serum phospholipids and synthesis of phosphatidylcholine by fetal lungs during late gestation, suggesting a possible relationship between maternal phospholipid metabolism and fetal lung maturation.
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Farrell PM, Avery ME (1975) Hyaline membrane disease. Am Rev Respir Dis 111: 657–658
Robert MF, Neff RK, Hubbell JP, Taeusch HW, Avery ME (1976) Association between maternal diabetes and the respiratory distress syndrome in the newborn. N Engl J Med 294: 357–360
Scholz RW, Woodward BM, Rhoades RA (1972) Utilization in vitro and in vivo of glucose and glycerol by rat lung. Am J Physiol 223: 991–996
Godinez RI, Longmore WJ (1973) Use of isolated perfused rat lung in studies on lung lipid metabolism. J Lipid Res 14: 138–144
Moxley MA, Longmore WJ (1977) Effect of experimental diabetes and insulin on lipid metabolism in the isolated perfused rat lung. Biochim Biophys Acta 488: 218–224
Maniscalco WM, Wilson CM, Gross I, Gobran L, Rooney SA, Warshaw JB (1978) Development of glycogen and phospholipid metabolism in fetal and newborn rat lung. Biochim Biophys Acta 530: 333–346
Gilden C, Sevanian A, Tierney DF, Kaplan SA, Barrett CT (1977) Regulation of fetal lung phosphatidylcholine synthesis by cortisol: role of glycogen and glucose. Pediatr Res 11: 845–858
Benito M, Lorenzo M, Madina JM (1982) Relationship between lipogenesis and glycogen synthesis in maternal and foetal tissues during late gestation in the rat. Effect of dexamethasone. Biochem J 204: 865–868
Bourbon JR, Rieutort M, Engle MJ, Farrell PM (1982) Utilization of glycogen for phospholipid synthesis in fetal rat lung. Biochim Biophys Acta 712: 382–389
Van Golde LMG (1976) Metabolism of phospholipids in the lung. Am Rev Respir Dis 114: 977–1000
Sanders RL, Longmore WJ (1975) Phosphatidylglycerol in rat lung. II. Comparison of occurrence, composition, and metabolism in surfactant and residual lung fractions. Biochemistry 14: 835–840
Gross I, Smith GJW, Wilson CM, Maniscalco WM, Ingleson LD, Brehier A, Rooney SA (1980) The influence of hormones on the biochemical development of fetal rat lung in organ culture. II. Insulin. Pediatr Res 14: 834–838
Sosenko IRS, Frantz ID, Roberts RI, Meyrick B (1980) Morphologic disturbance of lung maturation in fetuses of alloxan diabetic rabbits. Am Rev Respir Dis 122: 687–696
Rhoades RA, Filler DA, Vannata B (1979) Influence of maternal diabetes on lipid metabolism in neonatal rat lung. Biochim Biophys Acta 572: 132–138
Tyden O, Berne C, Eriksson U (1980) Lung maturation in fetuses of diabetic rats. Pediatr Res 14: 1192–1195
Tsai MY, Josephson MW, Brown DM (1981) Fetal rat lung phosphatidylcholine synthesis in diabetic and normal pregnancies. A comparison of prenatal dexamethasone treatments. Biochim Biophys Acta 664: 174–181
Demottaz V, Epstein MF, Frantz ID (1980) Phospholipid synthesis in lung slices from fetuses of alloxan diabetic rabbits. Pediatr Res 14: 47–49
Ofulue AF, Nijjar MS (1981) Influence of streptozotocin-induced diabetes on the cytoplasmic factors modulating adenylate cyclase activity in rat lungs. Biochim Biophys Acta 675: 397–402
Ofulue AF, Nijjar MS (1982) Effect of streptozotocin-induced diabetes on calmodulin and cyclic AMP phosphodiesterase activity in the rat lungs. Lung 160: 303–310
Nijjar MS (1979) Regulation of rat lung adenylate cyclase by cytoplasmic factor(s) during development. Biochim Biophys Acta 584: 43–50
Nijjar MS (1979) Role of cyclic AMP and related enzymes in rat lung growth and development. Biochim Biophys Acta 586: 464–472
Nijjar MS, Thurlbeck WM (1980) Alteration in enzymes related to adenosine 3′, 5′-monophosphate during compensatory growth of rat lung. Eur J Biochem 105: 403–407
Barrett CT, Sevanian A, Lavin N, Kaplan SA (1976) Role of adenosine 3′, 5′-monophosphate in maturation of fetal lungs. Pediatr Res 10: 621–625
Stahlmann MT, Gray ME, Lieu S, Chytel F (1974) The role of cyclic AMP in lamellar body synthesis and secretion. Pediatr Res 8: 470
Sohn RJ, Mathe AA, Volicer L (1977) Role of cyclic nucleotides in the normal lung and in bronchial asthma. In: Volicer L (ed) Clinical aspects of cyclic nucleotides, Spectrum Publications, New York, pp 193–227
Nijjar MS, Khanura BS, Juravsky LI (1982) Effect of maternal diabetes on the secretion of surfactant from the fetal and maternal rat lungs in vitro. Am Rev Respir Dis 125: 198 (Abstract)
Huggett ASG, Nixon DA (1957) Enzymatic determination of blood glucose. Biochem J 66: 12P
Folch J, Lees M, Sloane-Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497–509
Mason KJ, Nellenbogen J, Clements JA (1976) Isolation of disaturated phosphatidylcholine with osmium tetroxide. J Lipid Res 17: 281–284
Dixon WJ, Massey FJ (1957) The mean estimation and tests of hypothesis. In: Dixon WJ, Massey FJ (eds) Introduction to statistical analysis. McGraw-Hill, New York, pp 112–138
Glatz T, Ikegami M, Jobe A (1982) Metabolism of exogenously administered natural surfactant in the newborn lamb. Pediatr Res 6: 711–715
Biezenski JJ (1970) Role of placenta in fetal lipid metabolism. II. Phospholipid transfer in early rabbit gestation. Am J Obstet Gynecol 108: 638–642
Tsao FHC, Gutcher GR, Zachman RD (1979) Effect of hydrocortisone on the metabolism of phosphatidylcholine in maternal and fetal rabbit lungs and livers. Pediatr Res 13: 997–1001
Shafrir E, Khassis S (1982) Maternal-fetal fat transport versus new fat synthesis in the pregnant diabetic rat. Diabetologia 22: 111–117
Epstein MF, Farrell PM (1975) The choline incorporation pathway: primary mechanism for de novo lecithin synthesis in fetal primate lung. Pediatr Res 9: 658–665
Cunningham MD, Desai NS, Thompson SA, Green JM (1978) Amniotic fluid phosphatidylglycerol in diabetic pregnancies. Am J Obstet Gynecol 131: 719–724
Epstein MF, Farrell PM, Chez RA (1976) Fetal lung lecithin metabolism in the glucose-intolerant rhesus monkey pregnancy. Pediatrics 57: 722–728
Obenshain SS, Adam PAJ, King KC, Teramo K, Raivio KO, Raiha N, Schwartz R (1970) Human fetal insulin response to sustained maternal hyperglycemia. N Engl J Med 283: 566–570
Cuezva JM, Burnett ES, Kerr DS, Rodman HM, Patel MS (1982) The newborn of diabetic rat. 1. Hormonal and metabolic changes in the postnatal period. Pediatr Res 16: 632–637
Sosenko IR, Kitzmiller JL, Loo SW, Blix P, Rubenstein AH, Gabbay KH (1979) The infant of diabetic mother. Correlation of increased rd C-peptide levels with macrosomia and hypoglycemia. N Engl J Med 301: 859–862
Kim YS, Jatoi I, Kim Y (1980) Neonatal macrosomia in maternal diabetes. Diabetologia 18: 407–411
Fishler MC, Entenman C, Montgomery ML, Chaikoff IL (1943) The formation of phospholipid by the hepatectomized dog as measured with radioactive phosphorus. 1. The site of formation of plasma phospholipids. J Biol Chem 150: 47–55
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Nijjar, M.S., Khangura, B.S. & Juravsky, L.I. The effect of maternal diabetes on the synthesis and secretion of phosphatidylcholine in fetal and maternal rat lungs in vitro. Diabetologia 27, 219–224 (1984). https://doi.org/10.1007/BF00273810
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DOI: https://doi.org/10.1007/BF00273810