Summary
Three strains of ‘McIntosh’ apple (Malus domestica Borkh.) with growth habits ranging from the standard parent type to extremely compact (dwarf) were grown in vitro as meristem-tip cultures on Murashige and Skoog medium containing a range of concentrations of benzyladenine (BA). All strains exhibited a similar optima (3 to 6 μM BA) for maximum shoot proliferation and culture weight increase. However, tolerance to supra-optimal concentration of this cytokinin was related to growth habit. For example, at 10 μM BA shoot production rates as a percent of the maximum rates were 90%, 20% and zero for the extreme compact, moderate compact and standard strains, respectively. Comparisons among field trees and meristem-tip cultures of all three strains revealed similarities in growth and development.
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Communicated by P. Spiegel-Roy
Summerland Research Station, Contribution No. 532
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Lane, W.D., Looney, N.E. & Måge, F. A selective tissue culture medium for growth of compact (dwarf) mutants of apple. Theoret. Appl. Genetics 61, 219–223 (1982). https://doi.org/10.1007/BF00273778
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DOI: https://doi.org/10.1007/BF00273778