Summary
Interaction between R-factor determined streptomycin (Sm) resistance (R-Sm) and chromosomally determined Sm dependence (str-d) has been studied using three different approaches by (i) attempts to transfer by conjugation the R-factor into Sm-dependent recipients, (ii) transduction of str-d alleles into R + recipients, and (iii) investigation of Sm-dependent revertants of a suppressed Sm-dependent mutant harboring an R-factor. Evidence is presented which strongly suggests a lethal interference between the function of R-Sm genes and str-d allele determined Sm dependence in Proteus mirabilis.
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Abbreviations
- Sm:
-
streptomycin sulfate
- Cm:
-
chloramphenicol
- Su:
-
sulphathiacol
- Tc:
-
oxytetracycline
- str-s,-r,-d :
-
alleles of the str locus, determining Sm sensitivity, resistance, and dependence, respectively. Resistance genes carried by the R-genote are designed as R-Sm, R-Cm, R-Su, R-Tc.
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Communicated by F. Kaudewitz
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Hofemeister, J., Böhme, H. Interaction between R-factors and the host cells. Molec. Gen. Genet. 103, 258–265 (1968). https://doi.org/10.1007/BF00273697
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DOI: https://doi.org/10.1007/BF00273697