Summary
We have studied in cultured blood lymphocytes a familial translocation by banding with Giemsa staining, and have unequivocally identified the translocated chromosomes as Nos. 3 and 8, and besides we have established without doubt that the translocation has occurred between the short arm of chromosome No. 3 and the long arm of chromosome No. 8.
Zusammenfassung
Mit Hilfe von Bandenmustern, die mit Giemsafärbung zu erzielen sind, wurde eine familiäre Translokation in Lymphocytenkulturen untersucht. Die translozierten Chromosomen konnten eindeutig als Nr. 3 und Nr. 8 identifiziert werden; außerdem wurde zweifelsfrei festgestellt, daß die Translokation zwischen dem kurzen Arm von Nr. 3 und dem langen Arm von Nr. 8 erfolgt ist.
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References
Allderdice, P. M., Miller, O. J., Miller, D. A., Breg, W. R., Gendel, E., Zelson, C.: Familial translocation involving chromosomes 6, 14 and 20 identified by quinacrine fluorescence. Humangenetik 13, 205–209 (1971).
Amo, A. del, Gullón, A., Moncada, E., Bueno, M.: Relación de anomalias cariotípicas encontradas en 686 sujetos posibles poseedores de anomalias cromosómicas. Rev. Med. Univ. Nav. 15, 203–236 (1971).
Arakaki, D. T., Sparkes, R. S.: Microtechnique for culturing leukocytes from whole blood. Cytogenetics 2, 57–60 (1963).
Breg, W. R., Miller, O. J., Miller, D. A., Allderdice, P. W.: Distintive fluorescence of quinacrine-labelled human G group chromosomes. Nature New Biol. 231, 276–277 (1971).
Caspersson, T., Farber, S., Foley, G. E., Kudynowski, J., Modest, E. J., Simonsson, E., Wagh, U., Zech, L.: Chemical differentiation along metaphase chromosomes. Exp. Cell Res. 49, 219–222 (1968).
Caspersson, T., Lomakka, G., Zech, L.: The 24 fluorescence patterns of the human metaphase chromosomes-distinguishing characters and variability. Hereditas (Lund) 67, 89–102 (1971a).
Caspersson, T., Chapelle, A. de la, Lindsten, J., Schröder, J., Zech, L.: Absence of brightly fluorescent Y material in XX men. Ann. Génét. 14, 173–176 (1971b).
Caspersson, T., Hultén, M., Lindsten, J., Therkelsen, A. J., Zech, L.: Identification of different Robertsonian translocations in man by quinacrine mustard fluorescence analysis. Hereditas (Lund) 67, 213–220 (1971c).
Clarke, G., Stevenson, A. C., Davies, P., Williams, C. E.: A family apparently showing transmision of a translocation between chromosome 3 and one of the X-6-12 group. J. med. Genet. 1, 27–34 (1964).
Gropp, A., Marsh, W., Brodehl, J.: Structural (translocation) heterozygosity over three subsequent generations in man. Nature (Lond.) 207, 374–376 (1965).
Manolov, G., Manolova, Y., Levan, A., Klein, G.: Experiments with fluorescent chromosome staining in Kurkitt tumors. Hereditas (Lund) 68, 235–244 (1971).
O'Riordan, M. L., Robinson, J. A., Buckton, K. E., Evans, H. J.: Distinguishing between the chromosomes involved in Down's syndrome (trisomy 21) and chronic myeloid leukaemia (Ph1) by fluorescence. Nature (Lond.) 230, 167–168 (1971).
Patil, R. P., Merrick, S., Lubs, H. A.: Identification of each human chromosome with a modified Giemsa stain. Science 173, 821–822 (1971).
Ridler, M. A. C.: Banding patterns of metaphase chromosomes in Down's syndrome. Lancet 1971 II, 354–356.
Schnedl, W.: Banding pattern of human chromosomes. Nature New Biol. 233, 93–94 (1971).
Sumner, A. T., Evans, H. J., Buckland, R. A.: New technique for distinguishing between human chromosomes. Nature New Biol. 232, 31–32 (1971).
Wilson, M. G., Towner, J. W., Lipshin, J., Fleisher, A.: Identification fo an unusual Y chromosome in YY mosaicism by quinacrine fluorescence. Nature (Lond.) 231, 388–389 (1971).
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del Amo, A., Gullón, A. Familial translocation t(3q+;8q−) studied by banding with Giemsa staining. Hum Genet 15, 14–19 (1972). https://doi.org/10.1007/BF00273426
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DOI: https://doi.org/10.1007/BF00273426