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Additional electrophoretic variants of 6-phosphogluconate dehydrogenase

Summary

6 new PGD alleles are described. 2 of these (PGD Wantoat and PGD Canberra) control proteins that are electrophoretically slow: the other 4 control fast variants.

The heterozygotes PGD A/PGD Wantoat and PGD A/PGD Canberra give similar band spacing after starch gel electrophoresis, but the relative intensities of the bands in the 2 patterns is different.

Of the 4 fast variant alleles PGD Kadar controls a gene product with fastest mobility, PGD Caspian is slightly slower, whilst PGD Bombay and PGD Natal are similar in the mobility of their gene products, but differ in their reaction to treatment with 2-mercaptoethanol. After treatment with this reagent patterns involving PGD Natal revert to normal. None of the other gene products of the PGD alleles tested are affected by treatment with 2-mercaptoethanol.

PGD Kadar was found to be polymorphic in the tribal Kadar population of south India. 3 distinctive new patterns were found in this population representing the homozygote PGD Kadar/PGD Kadar, the heterozygote PGD A/PGD Kadar and the heterozygote PGD C/PGD Kadar.

Zusammenfassung

Es werden 6 neue PGD-Allele beschrieben. 2 davon (PGD Wantoat und PGD Canberra) sind Kontrollproteine, die elektrophoretisch langsam sind. Die 4 übrigen kontrollieren schnelle Variaten.

Die Heterozygote PGD A/PGD Wantoat und PGD A/PGD Canberra zeigen einen ähnlichen Abstand der Banden bei Stärkegelelektrophoresis, jedoch sind die relativen Intensitäten der Streifen in beiden Mustern verschieden.

Von den 4 schnellen Allelvarianten kontrolliert PGD Kadar ein Genprodukt mit schnellster Mobilität; PGD Caspian wirkt etwas langsamer, und sowohl PGD Bombay als auch PGD Natal zeigen eine ähnliche Wandlungsgeschwindigkeit, obwohl sie unter Behandlung mit 2-Mercaptoethanol verschieden reagieren. Nach Behandlung mit diesem Reagens werden Muster mit PGD Natal wieder normal. Keine der anderen Genprodukte, der PGD-Allele, die hier geprüft werden, werden durch Behandlung mit 2-Mercaptoethanol beeinflußt.

PGD Kadar stellte sich in der Kadar-Sippenbevölkerung Südindiens als polymorph heraus. 3 ausgeprägte neue Muster wurden in dieser Bevölkerung entdeckt, nämlich: der Homozygote PGD Kadar/PGD Kadar, der Heterozygote PGD A/PGD Kadar und der Heterozygote PGD C/PGD Kadar.

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Blake, N.M., Saha, N., McDermid, E.M. et al. Additional electrophoretic variants of 6-phosphogluconate dehydrogenase. Hum Genet 21, 347–354 (1974). https://doi.org/10.1007/BF00273373

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  • DOI: https://doi.org/10.1007/BF00273373

Keywords

  • Starch
  • Gene Product
  • Metabolic Disease
  • Relative Intensity
  • Variant Allele