Summary
With a view towards identifying new ATPase loci on the mitochondrial genome a large number of oligomycin-, ossamycin- and venturicidin-resistant mutants were isolated after MnCl2 mutagenesis. The mutants were subjected to mass-screens which divided them into different cross-resistance phenotype-classes and also distinguished the common OLI1 mutations from the mutations at all other loci.
Allelism tests between examples of the different classes of phenotype indicated that the majority of mutations in the population mapped at the previously known loci OLI1, OLI2, OLI3 and OLI4. Mutations conferring specific ossamycin resistance defined two new loci, namely OSS1 and OSS2 which are linked to the OLI2 and OLI1 loci respectively. A few rare mutations comprise a new locus OLI5 which is linked to the OLI1 locus (12.6% total recombination).
In conclusion we can now say that there are two unlinked segments of the mitochondrial genome, each of which is composed of several distinct, genetically-linked loci. One segment contains the OLI1, OLI3, OLI5 and OSS2 loci and the other the OLI2, OLI4 and OSS1 loci. The phenotypically-distinguishable mutations described herein should facilitate fine-structure mapping of these two segments.
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Avner, P.R., Coen, D., Dujon, B., Slonimski, P.P.: Mitochondrial genetics. IV. Allelism and mapping studies of oligomycin resistant mutants in S. cerevisiae. Mol. Gen. Genet. 125, 9–52 (1973)
Avner, P.R., Griffiths, D.E.: Studies on energy-linked reactions. Genetic analysis of oligomycin-resistant mutants of Saccharomyces cerevisiae. Eur. J. Biochem. 32, 312–321 (1973)
Clavilier, L.: Mitochondrial genetics. XII. An oligomycin-resistant mutant localized at a new mitochondrial locus in Saccharomyces cerevisiae. Genetics 83, 227–243 (1976)
Coen, D., Deutsch, J., Netter, P., Petrochilo, E., Slonimski, P.P.: Mitochondrial genetics. I. Methodology and phenomenology. Symp. Soc. Exp. Biol. 24, 449–496 (1970)
Colson, A.-M., Slonimski, P.P.: Genetic localization of diuron- and mucidin-resistant mutants relative to a group of loci of the mitochondrial DNA controlling coenzyme QH2-cytochrome c reductase in Saccharomyces cerevisiae. Mol. Gen. Genet. 167, 287–298 (1979)
Coruzzi, G., Trembath, M.K., Tzagoloff, A.: Assembly of the mitochondrial membrane system: Mutations in the pho2 locus of the mitochondrial genome of Saccharomyces cerevisiae. Eur. J. Biochem. 92, 279–287 (1978)
Coury, F., Tzagoloff, A.: Localization on mitochondrial DNA of mutations leading to a loss of rutamycin-sensitive adenosine triphosphatase. Eur. J. Biochem. 68, 113–119 (1976)
Griffiths, D.E., Houghton, R.L., Lancashire, W.E., Meadows, P.A.: Studies on energy-linked reactions: Isolation and properties of mitochondrial venturicidin-resistant mutants of Saccharomyces cerevisiae. Eur. J. Biochem. 51, 393–402 (1975)
Houghton, R.L., Lancashire, W.E., Griffiths, D.E.: A biochemical genetic investigation of oligomycin and related inhibitors. Biochem. Soc. Trans. 2, 210–213 (1974)
Kotylak, Z., Slonimski, P.P.: Fine structure genetic map of the mitochondrial DNA region controlling coenzyme QH2: cytochrome c reductase. In: Mitochondria 1977. Genetics and biogenesis of mitochondria, W. Bandlow, R.J., Schweyen, K. Wolf and F. Kaudewitz (eds.), pp. 161–172. Berlin and New York: Walter de Gruyter 1977
Lancashire, W.E., Griffiths, D.E.: Studies on energy-linked reactions: Isolation, characterisation and genetic analysis of trialkyltin-resistant mutants of Saccharomyces cerevisiae. Eur. J. Biochem. 51, 377–392 (1975a)
Lancashire, W.E., Griffiths, D.E.: Studies on energy-linked reactions: Genetic analysis of venturicidin-resistant mutants. Eur. J. Biochem. 51, 403–413 (1975b)
Lancashire, W.E., Houghton, R.L., Griffiths, D.E.: Two mitochondrial genes specifying venturicidin resistance in yeast. Biochem. Soc. Trans. 2, 213–215 (1974)
Lancashire, W.E., Mattoon, J.R.: Cytoduction: A tool for mitochondrial genetic studies in yeast. Utilization of the nuclear-fusion mutation karl-l for transfer of drug r and mit - genomes in Saccharomyces cerevisiae. Mol. Gen. Genet. 170, 333–344 (1979)
Lardy, H.A., Reed, P., Lin, C.-H.C.: Antibiotic inhibitors of mitochondrial ATP synthesis. Fed. Proc. 34, 1707–1710 (1975)
Putrament, A., Baranowska, H., Ejchart, A., Prazmo, W.: Manganese mutagenesis in yeast. A practical application of manganese for the induction of mitochondrial antibiotic-resistant mutations. J. Gen. Microbiol. 62, 265–270 (1975)
Putrament, A., Baranowska, H., Prazmo, W.: Induction by manganese of mitochondrial antibiotic resistance mutations in yeast. Mol. Gen. Genet. 126, 357–366 (1973)
Schweyen, R.J., Weiss-Brummer, B., Backhaus, B., Kaudewitz, F.: The genetic map of the mitochondrial genome, including the fine structure of cob and oxi clusters. In: Mitochondria 1977. Genetics and biogenesis of mitochondria, W. Bandlow, R.J., Schweyen, K., Wolf and F. Kaudewitz (eds.), pp. 138–148. Berlin and New York: Walter de Gruyter 1977
Tzagoloff, A., Meagher, P.: Assembly of the mitochondrial membrane system. VI. Mitochondrial synthesis of subunit proteins of the rutamycin-sensitive adenosine triphosphatase. J. Biol. Chem. 247, 594–603 (1972)
Wachter, E., Sebald, W., Tzagoloff, A.: Altered amino acid sequence of the DCCD-binding protein in the olil-resistant mutant D273-10B/A21 of Saccharomyces cerevisiae. In: Mitochondria 1977. Genetics and biogenesis of mitochondria, W. Bandlow, R.J. Schweyen, K. Wolf and F. Kaudewitz (eds.), pp. 441–450. Berlin and New York: Walter de Gruyter 1977
Walter, P., Lardy, H.A., Johnson, D.: Antibiotics as tools for metabolic studies. X. Inhibition of phosphoryl transfer reactions in mitochondria by peliomycin, ossamycin and venturicidin. J. Biol. Chem. 242, 5014–5018 (1967)
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Communicated by F. Kaudewitz
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Lancashire, W.E., Mattoon, J.R. Genetics of oxidative phosphorylation: mitochondrial loci determining ossamycin-, venturicidin- and oligomycin-resistance in yeast. Molec. Gen. Genet. 176, 255–264 (1979). https://doi.org/10.1007/BF00273220
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DOI: https://doi.org/10.1007/BF00273220