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Detection of mycoplasma contamination in tissue cultures by fluorescence microscopy

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Summary

The in situ staining method of Chen (1977) for the detection of mycoplasma contaminants in tissue cultures was tested in cultures of human skin fibroblasts after controlled contamination with Mycoplasma arginini. It is concluded that this method is reliable only at infection rates of 100% or higher, i.e., at one mycoplasma or more per tissue-culture cell.

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References

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Authors and Affiliations

  1. Institute of Human Genetics, Bartholin Bygningen, University of Århus, DK-8000, Aarhus C, Denmark

    Maya Thangavelu, H. Ernø & A. J. Therkelsen

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  1. Maya Thangavelu
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  2. H. Ernø
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  3. A. J. Therkelsen
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Thangavelu, M., Ernø, H. & Therkelsen, A.J. Detection of mycoplasma contamination in tissue cultures by fluorescence microscopy. Hum Genet 47, 199–202 (1979). https://doi.org/10.1007/BF00273202

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  • DOI: https://doi.org/10.1007/BF00273202

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