Summary
This investigation deals with the possibility and the attempt to find a reliable method for AB0-typing of ancient bones. Using the Ouchterlonytest and the Immunorheophoresis we got satisfying reactions. Typing 169 femora from the 15th to 17th centuries from the Charnel house in Oppenheim, the following results were obtained: 36.2% A, 22.8% B, 10.1% AB and 30.9% H. 20 femora (11.8%) did not react at all. Presumably these samples belonged to nonsecretors who possess no blood group active material in their secretions and tissues. Compared with the present distribution of ABH-phenotypes and alleles the allele B showed a higher percentage.
Zusammenfassung
Diese Untersuchung befaßt sich mit der Möglichkeit und dem Versuch, eine objektive Methode für den AB0-Nachweis an historischem Knochenmaterial zu finden. Mit dem Ouchterlonytest und der Immunorheophorese gelang es, zuverlässige Ergebnisse zu erhalten. Zum Test gelangten 169 Femora aus dem Beinhaus von Oppenheim. Sie stammen aus dem 15.–17. Jahrhundert. 36,2% zeigten die Gruppe A, 22,8% Gruppe B, 10,1% Gruppe AB und 30,9% Gruppe H. von den 169 Femora erbrachten 20 (11,8%) keine Reaktion. Vermutlich handelt es sich hier um Proben von Nichtsekretoren, die keine blutgruppenaktiven Substanzen in ihren Sekreten und Geweben besitzen. Verglichen mit der heutigen AB0-Verteilung in diesem Raum ist das Allel B erhöht.
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References
Bird, G. W. G.: Agar gel studies of blood group specific substances and precipitins of plant origin. I, II. Vox Sang. (Basel) 4, 307, 313 (1959).
Borgognini, S. M.: Dimostrazione della presenza di alcuni costituenti fondamentali delle sostance gruppo specifiche AB0 in ossa di diversa antichita. Atti Soc. Tosc. Sci. Nat. Mem. Serie B 75, 202 (1968).
—: New trends in blood group determination in human bones. Proc. VIIIth Intern. Congr. Anthrop. Ethnol. Sci., Tokyo-Kyoto 1968, p. 114. Science Council of Japan: Tokyo 1969.
Borgognini, S. M.: Blood group determination of 33 bone samples from the early medieval cemetry of Gàva Màrket. Anthropologiai Közlemények (in press).
Borgognini, S. M., Bartoloni, S., Omer, C.: Determinazione dei gruppi sanguini AB0 in un gruppo di scheletri eneolitici proveniente dalla necropoli di Ponte S. Pietro Arch. Anthrop. Etnol. 97, 35 (1967).
Boyd, W. C., Boyd, L. G.: An attempt to determine the blood groups of mummies. Proc. Soc. exp. Biol. (N.Y.) 31, 671 (1934).
Candela, P. B.: Blood group reactions in ancient human skeletons. Amer. J. Phys. Anthrop. 21, 429 (1936).
Coons, A. H., Greech, H. J., Jones, R. N., Berliner, E.: The demonstration of pneumococcal antigen in tissues by use of fluorescent antibody. J. Immunol. 45, 159 (1942).
Harsányi, L., Geréncsér, G.: Nachweis von Gruppensubstanzen in Geweben mit der “Mixed Cell Agglutination”-Methode. Dtsch. Z. ges. gerichtl. Med. 63, 162 (1968).
Kout, M., Vacikova, A., Stloukal, M.: An attempt to assess blood groups in palaeoanthropological material. Anthropologie 11/3, 49 (1965).
Lengyel, I., Nemeskeri, J.: The application of biochemical methods to biological reconstruction. Z. Morph. Anthrop. 54, 1 (1963).
Lippold, L. K.: The mixed cell agglutination method for typing mummified human tissue. Amer. J. Phys. Anthrop. 34, 377 (1971).
Morgan, W. T. J., Watkins, W. M.: Genetic and biochemical aspects of human blood group A-, B-, H-, Lea-and Leb-specificity. Brit. med. Bull. 25, 30 (1969).
Oss, C. J. van, Bronson, P. M.: Immunorheophoresis. Immunochem. 6, 775 (1969).
Pardini, E.: Determinazione del contenuto in N-Acetilglucoso e galattosamine in ossa umane antiche e recente. Atti Soc. Tosc. Sci. Nat. Mem. Serie B 75, 1 (1968).
Salazar Mallen, M.: Estudio immunologico de restos oseos antiguos. Gac. méd. Méx. 81, 122 (1951).
Thieme, F. P., Otten, C. M.: The unreliability of blood typing aged bone. Amer. J. Phys. Anthrop. 15, 387 (1957).
—, Sutton, H. E.: A blood typing of human skull fragments from the Pleistocene. Amer. J. Phys. Anthrop. 14, 437 (1956).
Vogel, F.: AB0 blood groups and disease. Amer. J. hum. Genet. 22, 464 (1970).
— Pettenkofer, H. J., Helmbold, W.: Über die Populationsgenetik der AB0-Blutgruppen. Acta genet. (Basel) 10, 267 (1960).
Watkins, W. M., Koscielak, J., Morgan, W. T. J.: The relationship between the specificity of the blood group A and B substances isolated from erythrocytes and from secretions. Proc. 9th Congr. int. Soc. Blood Transf., Mexico 1962, p. 213. Basel: Karger 1964.
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Kellermann, G. Methodological investigations on the AB0-Typing of ancient bones. Hum Genet 14, 50–55 (1971). https://doi.org/10.1007/BF00273031
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DOI: https://doi.org/10.1007/BF00273031