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A rapid transformation method for Solanum tuberosum using binary Agrobacterium tumefaciens vectors

Plant Cell Reports volume 7, pages 13–16 (1988)Cite this article

Abstract

A tuber disc transformation and regeneration system was devised for potato (Solanum tuberosum). Tuber discs were found to be the most morphogenetic organ on a medium previously optimised for tomato regeneration. Shoot regeneration from tuber discs was rapid and transformed as shown by nopaline assays and Southern blot analysis. The ease and speed of the tuber disc method will allow for the increased use of this commercially important plant in transformation studies.

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Abbreviations

MS:

Murashige and Skoog medium

IAA:

indole 3-acetic acid

BAP:

benzylaminopurine

NAA:

naphthalene acetic acid

ZR:

zeatin riboside

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Affiliations

  1. Molecular Genetics Department, Institute of Plant Science Research (Cambridge Laboratory), Maris Lane, Trumpington, CB2 2LQ, Cambridge, UK

    S. Sheerman & M. W. Bevan

Authors
  1. S. Sheerman
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  2. M. W. Bevan
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Communicated by J. Schell

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Sheerman, S., Bevan, M.W. A rapid transformation method for Solanum tuberosum using binary Agrobacterium tumefaciens vectors. Plant Cell Reports 7, 13–16 (1988). https://doi.org/10.1007/BF00272967

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  • DOI: https://doi.org/10.1007/BF00272967

Keywords

  • Blot Analysis
  • Southern Blot
  • Regeneration System
  • Southern Blot Analysis
  • Solanum Tuberosum