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Intergeneric somatic hybrid plants of Citrus sinensis cv. Hamlin and Poncirus trifoliata cv. Flying Dragon

Abstract

Intergeneric somatic hybrid plants between ‘Hamlin’ sweet orange [Citrus sinensis (L.) Osbeck] and ‘Flying Dragon’ trifoliate orange (Poncirus trifoliata Raf.) were regenerated following protoplast fusion. ‘Hamlin’ protoplasts, isolated from an habituated embryogenic suspension culture, were fused chemically with ‘Flying Dragon’ protoplasts isolated from juvenile leaf tissue. The hybrid selection scheme was based on complementation of the regenerative ability of the ‘Hamlin’ protoplasts with the subsequent expression of the trifoliate leaf character of ‘Flying Dragon.’ Hybrid plants were regenerated via somatic embryogenesis and multiplied organogenically. Hybrid morphology was intermediate to that of the parents. Chromosome counts indicated that the hybrids were allotetraploids (2n=4x=36). Malate dehydrogenase (MDH) isozyme patterns confirmed the hybrid nature of the regenerated plants. These genetically unique somatic hybrid plants will be evaluated for citrus rootstock potential. The cell fusion, selection, and regeneration scheme developed herein should provide a general means to expand the germplasm base of cultivated Citrus by intergeneric hybridization with related sexually incompatible genera.

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Abbreviations

MDH:

malate dehydrogenase

CTV:

citrus tristeza virus

MT:

Murashige and Tucker basal medium

BH3:

protoplast culture medium, Grosser and Chandler, 1987

PEG:

polyethylene glycol

GA3:

giberellic acid

BA:

N-(phenylmethyl)-1 H-purin-6-amine

HCl:

hydrochloric acid

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Communicated by G. C. Phillips

Florida Agricultural Experiment Station Journal Series No. 7972

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Grosser, J.W., Gmitter, F.G. & Chandler, J.L. Intergeneric somatic hybrid plants of Citrus sinensis cv. Hamlin and Poncirus trifoliata cv. Flying Dragon. Plant Cell Reports 7, 5–8 (1988). https://doi.org/10.1007/BF00272965

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  • DOI: https://doi.org/10.1007/BF00272965

Keywords

  • Somatic Embryogenesis
  • Malate Dehydrogenase
  • Hybrid Plant
  • Protoplast Fusion
  • Sweet Orange