Summary
We have investigated the influence of bacterial DNA replication on the formation of infectious and transducing Mu-particles.
The data obtained agree with the previous findings that growth of phage Mu is independent of the host dnaA gene product (Toussaint and Faelen 1974), but requires bacterial replication forks (Fitts and Taylor 1980). The replication of transducting DNA during phage development (Teifel and Schmieger 1979) is controlled by the host and is not a precondition for its packaging. Packaging of transducing DNA does not require a nearby Mu genome.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Backhaus H, Schmieger H (1979) Replication and maturation of phage P22 in a mutant of Salmonella typhimurium temperature sensitive in initiation of DNA replication. Mol Gen Genet 171:295–299
Bade EG, Howe MM, Rawluk L (1978) Preferential generalized transduction by bacteriophage Mu. Mol Gen Genet 160:89–94
Bukhari AI, Ljungquist E (1977) Bacteriophage Mu: Methods for cultivation and use. In: Bukhari AI, Shapiro JA, Adhya SL (eds) DNA insertion elements, plasmids and episomes. Cold Spring Harbor Press, New York, pp 749–756
Bukhari AI, Taylor AL (1975) Interence of insertions on the packaging of host sequences covalently linked to bacteriophage Mu DNA. Proc Natl Acad Sci USA 72:4399–4403
Chelala CA, Margolin P (1974) Effects of deletions on cotransduction linkage in Salmonella typhimurium: Evidence that bacterial chromosome deletions affect the formation of transducing DNA fragments. Mol Gen Genet 131:97–112
Daniell E, Robberts R, Abelson J (1972) Mutations in the lactose operon caused by bacteriophage Mu. J Mol Biol 69:1–8
Fitts RA, Taylor AL (1980) Integration of bacteriophage Mu at host chromosomal replication forks during lytic development. Proc Natl Acad Sci USA 77:2801–2805
Hirota Y, Mordoh J, Jacob F (1970) On the process of cellular division in Escherichia coli. III. Thermosensitive mutants of E. coli altered in the process of DNA initiation. J Mol Biol 53:369–388
Howe MM (1973) Transduction by bacteriophage Mu-1. Virology 55:103–117
Nishimura Y, Caro L, Berg CM, Hirota Y (1971) Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome. J Mol Biol 55:441–456
Paolozzi L, Ghelardini P, Kepes A, Marcovich H (1979) The mechanism of integration of phage Mu in the chromosome of E. coli. Biochem Biophys Res Commun 88:111–116
Pato ML, Waggoner BT (1981) Cellular location of Mu DNA replicas. J Virol 38:249–255
Schröder W, Bade EG, Delius H (1974) Participation of E. coli DNA in the replication of temperate phage Mu-1. Virology 60:534–542
Teifel J, Schmieger H, (1979) The origin of the DNA in transducing particles of bacteriophage Mu. Density gradient analyses of intact phages. Mol Gen Genet 176:293–295
Teifel J, Schmieger H (1981) The origin of the DNA in transducing particles of bacteriophage Mu. Density gradient analyses of DNA. Mol Gen Genet 184:312–313
Toussaint A, Faelen M (1974) The dependence of temperate phage Mu-1 upon replication functions of E. coli K12. Mol Gen Genet 131:209–214
Zipser D, Bukhari AI (1972) Random insertion of Mu-1 DNA within a single gene. Nature New Biol 236:240–243
Author information
Authors and Affiliations
Additional information
Communicated by H. Böhme
Rights and permissions
About this article
Cite this article
Teifel, J., Schmieger, H. The influence of host DNA replication on the formation of infectious and transducing Mu-particles. Molec. Gen. Genet. 184, 308–311 (1981). https://doi.org/10.1007/BF00272922
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00272922