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Expression of streptococcal plasmid-determined resistance to erythromycin and lincomycin in Escherichia coli

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Summary

By using recombinant DNA techniques, we have constructed a chimeric plasmid, pSM7322 (10.8 kb), between the streptococcal erythromycin resistance vector plasmid pSM7 (6.4 kb) and the E. coli vector pBR322 (4.4 kb). As judged by the minimum inhibitory concentrations of erythromycin and lincomycin, pSM7-determined resistance to these antibiotics is expressed in E. coli.

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Authors and Affiliations

  1. Department of Clinical Bacteriology, University of Umeå, S-90185, Umeå, Sweden

    Horst Malke & Stig E. Holm

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  1. Horst Malke
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  2. Stig E. Holm
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Communicated by H. Böhme

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Malke, H., Holm, S.E. Expression of streptococcal plasmid-determined resistance to erythromycin and lincomycin in Escherichia coli . Molec. Gen. Genet. 184, 283–285 (1981). https://doi.org/10.1007/BF00272918

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  • DOI: https://doi.org/10.1007/BF00272918

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