Summary
In vitro binding of RNA polymerase to DNA of the minichromosome pCM959 revealed 5 binding sites, one within the origin of replication, oriC. A comparison of the location of the binding sites with the nucleotide sequence allowed the definition of 4 promoters, the transcripts of which allow the synthesis of 4 polypeptides.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bazaral M, Helinski DR (1968) Circular DNA forms of colicinogenic factors E1, E2 and E3 from E. coli. J Mol Biol 36:185–194
Buhk HJ (1981) Analyse cines Minichromosomes von E. coli. Ph.D. thesis, FU Berlin
Burgess RR, Jendrisak JJ (1975) A procedure for the rapid, large-scale purification of E. coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography. Biochemistry 14:4634–4638
Clewell DB (1972) Nature of ColE1 plasmid replication in E. coli in the presence of chloramphenicol. J Bacteriol 110:667–676
Katz L, Kingsbury DT, Helinski DR (1973) Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex. J Bacteriol 114:577–591
Koller T, Sogo JM, Bujard H (1974) An electron microscopic method for studying nucleic acid protein complexes. Visualization of RNA polymerase bound to the DNA of bacteriophages T7 and T3. Biopolymers 13:995–1009
Konings RNH, Schoenmakers JGG (1978) Transcription of the filamentous phage genome. In: DT Denhardt, D Dressler, DS Ray (eds) The Single-Stranded DNA Phages. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., pp 507–530
Lark KG (1972) Evidence for direct involvement of RNA in the initiation of DNA replication in E. coli 15T-. J Mol Biol 64:47–60
Leonard AC, Weinberger M, Munson BR, Helmstetter CE (1980) The effects of oriC containing plasmids on host cell growth. ICNUCLA Symp Mol Cell Biol 19:171–180
Lescure B, Oudet P, Chambon P, Yaniv M (1976) Transcription of polyoma virus DNA in vitro. Localization of E. coli RNA polymerase initiation sites. J Mol Biol 108:83–97
Lother H, Buhk HJ, Morelli B, Heimann B, Chakraborty T, Messer W (1981) Genes, transcriptional units and functional sites in and around the E. coli replication origin. ICN-UCLA Symp Molec Cell Biol 21 (in press)
Meijer M, Beck E, Hansen FG, Bergmans HEN, Messer W, v. Meyenburg K, Schaller H (1979) Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome. Proc Natl Acad Sci USA 76:580–584
Meijer M, Messer W (1980) Functional analysis of minichromosome replication: Bidirectional and unidirectional replication from the Escherichia coli replication origin, oriC. J Bacteriol 143:1049–1053
Messer W (1972) Initiation of DNA replication in E. coli B/r. Chronology of events and transcriptional control of initiation. J Bacteriol 112:7–12
Messer W, Bergmans HEN, Meijer M, Womack JE, Hansen FG, v. Meyenburg K (1978) Mini-chromosomes: Plasmids which carry the E. coli replication origin. Mol Gen Genet 162:269–275
v. Meyenburg K, Hansen FG (1980) The origin of DNA replication, oriC, of the Escherichia coli chromosome: Genes near oriC and construction of oriC deletion mutants. ICN-UCLA Symp Mol and Cell Biol 19:137–159
v. Meyenburg K, Hansen FG, Riise E, Bergmans HEN, Meijer M, Messer W (1979) Origin of replication, oriC, of the Escherichia coli K-12 chromosome: Genetic mapping and minichromosome replication. Cold Spring Harbor Symp Quant Biol 43:121–128
Miki T, Hiraga S, Nagata T, Yura T (1978) Bacteriophage λ carrying the E. coli chromosomal region of the replication origin. Proc Natl Acad Sci USA 75:5099–5103
Portmann R, Koller T (1976) The divalent cation method for proteinfree spreading of nucleic acid molecules. Sixth European Congress Electron Microscopy Jerusalem, Tal International Publishing Company. Vol 2, pp 546–548
Pribnow D (1975) Bacteriophate T7 early promoters: Nucleotide sequences of two RNA polymerase binding sites. J Mol Biol 99:419–443
Rosenberg M, Court D (1979) Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet 13:319–353
Shine J, Dalgarno L (1974) The 3′-terminal sequence of E. coli 16 S ribosomal RNA: Complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci USA 71:1342–1340
Siebenlist U, Simpson RB, Gilbert W (1980) E. coli RNA polymerase interacts homologous with two different promoters. Cell 20:269–281
Sogo JM, Inciarte MR, Corral J, Vinuela E, Salas M (1979) RNA polymerase binding sites and transcription map of the DNA of Bacillus subtilis phage Φ29. J Mol Biol 127:411–436
Steitz JA (1980) RNA-RNA interactions during polypeptide chain elongation. In: Chambliss G (ed) Ribosomes, structure, function, and genetics. University Park Press, Baltimore
Stüber D, Bujard H, (1981) The organization of transcriptional signals in plasmids pBR322 and pACYC184. Proc Natl Acad Sci USA 78:167–171
Stüber D, Delius H, Bujard H (1978) Electron microscopic analysis of in vitro transcriptional complexes: Mapping of promoters of the coliphage T5 genome. Mol Gen Genet 166:141–149
Sugimoto K, Oka A, Sugisaki H, Takanami M, Nishimura A, Yasuda Y, Hirota Y (1979) Nucleotide sequence of Escherichia coli, replication origin. Proc Natl Acad Sci USA 76:575–579
Yasuda S, Hirota Y (1977) Cloning and mapping of the replication origin of Escherichia coli. Proc Natl Acad Sci USA 74:5458–5462
Zyskind JW, Deen LT, Smith DW (1979) Isolation and mapping of plasmids containing the S. typhimurium origin of DNA replication. Proc Natl Acad Sci USA 76:3079–3101
Author information
Authors and Affiliations
Additional information
Communicated by E. Bautz
Rights and permissions
About this article
Cite this article
Morelli, G., Buhk, HJ., Fisseau, C. et al. Promoters in the region of the E. coli replication origin. Molec. Gen. Genet. 184, 255–259 (1981). https://doi.org/10.1007/BF00272913
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00272913