Summary
Tn951 is flanked by two perfect inverted repeats of 41 bp which include the 38 bp sequence of the IR of Tn3. Tn951 also contains the last 100 bp of the tnpA gene but with at least two mutations. However, beyond nucleotide 137 the sequences diverge and hybridization experiments show that Tn951 lacks at least the first two thirds of the tnpA gene.
In agreement with these observations Tn951 does not transpose by itself at a detectable frequency but can be complemented by the tnpA gene of Tn801 or Tn3. Tn501, Tn1721 and gamma delta do not complement Tn951 transposition.
Transposition of Tn951 duplicates 5 bp of target DNA sequence.
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Communicated by E. Bautz
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Cornelis, G., Sommer, H. & Saedler, H. Transposon Tn951 (TnLac) is defective and related to Tn3. Molec. Gen. Genet. 184, 241–248 (1981). https://doi.org/10.1007/BF00272911
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DOI: https://doi.org/10.1007/BF00272911