Summary
The efficiency of dihaploid production from anther culture in N. rustica has been improved by studying the effects of pretreatment temperature, pretreatment duration and initial anther stage on anther response, anther productivity and time to first plantlet production. Pretreatment was most effective on anthers at or around the stage of pollen mitosis. Pollen mitosis stage anthers pretreated at 9 °C for 15 days gave the best results. Both spontaneous and induced dihaploids were obtained. Small plantlets treated with 0.4% colchicine and 2% DMS solution for 5 h produced the maximum number of dihaploids (more than 50%). These considerable improvements in the efficiency of the techniques have made dihaploidy an attractive method for producing inbred lines in N. rustica. This will permit a large scale comparison of dihaploids with more conventional methods of inbreeding such as single seed descent and pedigree breeding.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brown JS, Wernsman EA (1982) Nature of reduced productivity of anther-derived dihaploid lines of flue-cured tobacco. Crop Sci 22:1–5
Burk LG, Gwynn GR, Chaplin JF (1972) Diploidized haploids from aseptically cultured anthers of Nicotiana tabacum. J Hered 63:355–360
Burk LG, Matzinger DF (1976) Variation among anther-derived doubled haploids from an inbred line of tobacco. J Hered 67:381–384
Chowdhury MKU (1982) The production of homozygous dihaploids from Nicotiana tabacum and N. rustica through anther culture. MSc Thesis, University of Birmingham, UK
De Paepe R, Bleton D, Grangbe R (1981) Basis and extent of genetic variability among doubled haploid plants obtained by pollen culture in Nicotiana sylvestris. Theor Appl Genet 59:177–184
Hlasnikova A (1977) Androgenesis in vitro evaluated from the aspects of Genetics. Z Pflanzenzücht 78:44–56
Jensen CJ (1974) Chromosome doubling techniques in haploids. In: Kasha KJ (ed) Haploids in higher plants. University of Guelph, Guelph, pp 153–190
Kasperbauer MJ, Collins GB (1972) Reconstitution of diploids from leaf tissue of anther derived haploids in tobacco. Crop Sci 12:98–101
Mather K, Vines A (1952) The inheritance of height and flowering time in a cross of Nicotiana rustica. In: Reeve ECR, Waddington CN (eds) Quantitative inheritance. HMSO, London, pp 49–80
Nakamura A, Yamada T, Kadotani N, Itagaki R, Oka M (1974) Studies on the haploid method of breeding in tobacco. SABRAO 6:107–131
Nitsch JP (1969) Experimental androgenesis in Nicotiana. Phytomorphology 19:389–404
Oinuma T, Yoshida T (1974) Genetic variation among doubled haploid lines of burley tobacco varieties. Jpn J Breed 24:211–216
Sunderland N (1974) Anther culture as a means of haploid induction. In: Kasha KJ (ed) Haploids in higher plants. University of Guelph, Guelph, pp 91–122
Sunderland N, Roberts M (1979) Cold-pretreatment of excised flower buds in float culture of tobacco anthers. Ann Bot 43:405–414
Tomes DT, Collins GB (1976) Factors affecting haploid plant production from in vitro anther cultures of Nicotiana species. Crop Sci 16:837–840
Author information
Authors and Affiliations
Additional information
Communicated by R. Riley
Rights and permissions
About this article
Cite this article
Chowdhury, M.K.U. An improved method for dihaploid production in Nicotiana rustica through anther culture. Theoret. Appl. Genetics 69, 199–204 (1984). https://doi.org/10.1007/BF00272895
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00272895