Skip to main content
Log in

Nicotiana chloroplast genome

7. Expression in E. coli and B. subtilis of tobacco and Chlamydomonas chloroplast DNA sequences coding for the large subunit of RuBP carboxylase

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

RuBPCase, the enzyme responsible for carboxylation and oxidation of RuBP in a wide variety of photosynthetic organisms, is the major protein found in the chloroplast. Here we present the first evidence for direct expression in E. coli and B. subtilis of tobacco and Chlamydomonas ct-DNA sequences coding for the LS of RuBPCase as demonstrated by a simple in situ immunoassay.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Anderson D, Shapio L, Skalka AM (1980) In situ Immunoassays for translation products. Methods Enzymol 68:428–437

    Google Scholar 

  • Bolivar F, Backman K (1979) Plasmids of E. coli as cloning vectors. Methods Enzymol 68:245–267

    Google Scholar 

  • Bottomley W, Whitfeld PR (1978) The products of cell-free transcription and translation of total spinach chloroplast DNA In: Akoyunoglou G (ed) Chloroplast development. Elsevier, Amsterdam, pp 657–660

    Google Scholar 

  • Chan PH, Wildman SG (1972) Chloroplast DNA codes for the primary of the large subunit of fraction I protein. Biochim Biophys Acta 227:677–680

    Google Scholar 

  • Chua NH, Schmidt GW (1978) Post-translational transport into intact chloroplast of a precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase. Proc Natl Acad Sci USA 75:6110–6114

    Google Scholar 

  • Coen DM, Bedbrook JR, Bogorad L, Rich A (1977) Maize chloroplast DNA fragment encoding the large subunit of ribulose bisphosphate carboxylase. Proc Natl Acad Sci USA 74:5487–5491

    Google Scholar 

  • Dalgart M, Ehrlich SD (1979) Prolonged incubation in calcium chloride improves the competence of E. coli cells. Gene 6:23–28

    Google Scholar 

  • Erion JL, Tarnowski J, Weissbach H, Brot N (1981) Cloning, mapping, and in vitro transcription-translation of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase from spinach chloroplasts. Proc Natl Acad Sci USA 78:3459–3463

    Google Scholar 

  • Gatenby AA, Castleton JA, Saul MW (1981) Expression in E. coli of maize and wheat chloroplast genes for large subunit of ribulose bisphosphate carboxylase. Nature 291:117–121

    Google Scholar 

  • Gray JC, Kekwick RGO (1973) Synthesis of the small subunit of ribulose-1,5-diphosphate carboxylase on cytoplasmic ribosomes from green leaves. FEBS Lett 38:67–69

    Google Scholar 

  • Herrmann RG, Whitfeld PR, Bottomley W (1980) Construction of a SalI/PstI restriction map of spinach chloroplast DNA using low-gelling temperature-agarose electrophoresis. Gene 8:179–191

    Google Scholar 

  • Highfield PE, Schmidt GW (1978) Synthesis and transport of the small subunit of chloroplast ribulose bisphosphate carboxylase. Nature 271:420–425

    Google Scholar 

  • Jensen KG, Bahr JT (1977) Ribulose 1,5-bisphosphate carboxylase-oxygenase. Annu Rev Plant Physiol 28:379–478

    Google Scholar 

  • Kolodner R, Tewari KK (1975) The molecular size and conformation of the chloroplast DNA from higher plants. Biochim Biophys Acta 402:372–390

    Google Scholar 

  • Kung SD (1977) Expression of chloroplast genomes in higher plants. Annu Rev Plant Physiol 28:401–437

    Google Scholar 

  • Lovett PS, Keggins KM (1979) Bacillus subtilis as a host for molecular cloning. Methods Enzymol 68:342–357

    Google Scholar 

  • Malnoë P, Rochaix JD, Chua NH, Spahr PF (1979) Characterization of the gene and messenger RNA of the large subunit of ribulose-1,5-diphosphate carboxylase in Chlamydomonas reinhardii J Mol Biol 133:417–434

    Google Scholar 

  • Maniatis T, Jeffrey A, Kleid DG (1975) Nucleotide sequence of right-ward operator of phage. Proc Natl Acad Sci USA 72:1184–1188

    Google Scholar 

  • Rhodes PG, Kung SD (1981) Chloroplast DNA isolation: purity achieved without nuclease digestion. Can J Biochem 59:911–915

    Google Scholar 

  • Smith GE, Summers MD (1980) The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper. Anal Biochem 109:123–129

    Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517

    Google Scholar 

  • Williams DM, Schoner RG, Duvall EJ, Preis LH, Lovett PS (1981) Expression of Escherichia coli trp genes and the mouse dihydrofolate reductase gene cloned in Bacillus subtilis. Gene 16:199–206

    Google Scholar 

  • Zhu YS, Duvall EJ, Lovett PS, Kung SD (1982) Nicotiana chloroplast genome. V. Construction, mapping and expression of clone library of N. otophora chloroplast DNA. Mol Gen Genet 187:61–66

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by D. von Wettstein

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhu, Y.S., Lovett, P.S., Williams, D.M. et al. Nicotiana chloroplast genome. Theoret. Appl. Genetics 67, 333–336 (1984). https://doi.org/10.1007/BF00272870

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00272870

Key words

Navigation