Summary
The organization of plant cells (or any other genetic elements, such as organelles) into aggregates modifies the expected distribution of mutants in clones. The reason for the modification, and its effect on the use of the Luria-Delbrück fluctuation test, are discussed. The Luria-Delbriick test was used to show that the trait for chlorate resistance in cultured rose-cell aggregates appeared spontaneously and in the absence of chlorate ion.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature
Crump, K.S.; Hoel, D.G. (1974): Mathematical models for estimating mutation rates in cell populations. Biometrika 61, 237–252
Dujon, B.; Bolotin-Fukuhara, M.; Coen, D.; Deutsch, J.; Netter, P.; Slonimski, P.P.; Weill, L. (1976): Mitochondrial genetics XI. Mutations at the mitochondrial locus w affecting the recombination of mitochondrial genes in Saccharomyces cerevisiae. Mol. Gen. Genet. 143, 131–165
Gillham, N.W. (1974): Genetic analysis of the chloroplast and mitochondrial genomes. Ann. Rev. Genet. 8, 347–391
Gillham, N.W.; Levine, P.P. (1962): Studies on the origin of streptomycin resistant mutants in Chlamydomonas reinhardi. Genetics 47, 1463–1474
Luria, S.E.; Delbrück, M. (1943): Mutations of bacteria from virus sensitivity to virus resistance. Genetics 28, 491–511
Maliga, P. (1978): Resistance mutants and their use in genetic manipulation. In: Frontiers of Plant Tissue Culture, 1978, Proc. 4th. Intl. Congr. Plant Tissue and Cell Culture (ed. Thorpe, T.), pp. 381–392. Calgary, Canada: University of Calgary
Massey, F.J., Jr. (1951): The Kolmogorov-Smirnov test for goodness of fit. J. Am. Statist. Assoc. 46, 68–78
Meins, F., Jr.; Binns, A. (1977): Epigenetic variation of cultured somatic cells: evidence for gradual changes in the requirement for factors promoting cell division. Proc. Nat. Acad. Sci. (USA) 74, 2928–2932
Murphy, T.M.; Hamilton, C.M.; Street, H.E. (1979): A strain of Rosa damascene resistant to ultraviolet light. Plant Physiol. 64, 936–941
Murphy, T.M.; Imbrie, C.W. (1981): Induction and characterization of chlorate-resistant strains of Rosa damascena cultured cells. Plant Physiol. 67, 910–916
Sager, R. (1962): Streptomycin as a mutagen for nonchromosomal genes. Proc. Nat. Acad. Sci. (USA) 48, 2018–2026
Street, H.E. (1977): Plant Tissue and Cell Culture. Berkeley: Univ. Calif. Press
Widholm, J.M. (1977): Selection and characterization of biochemical mutants. In: Plant Tissue Culture and Its Bio-Technological Application (eds. Barz, W.; Reinhard, E.; Zenk, M.H.). Berlin — Heidelberg — New York: Springer
Author information
Authors and Affiliations
Additional information
Communicated by L. Alföldi
Rights and permissions
About this article
Cite this article
Murphy, T.M. Analysis of distributions of mutants in clones of plant-cell aggregates. Theoret. Appl. Genetics 61, 367–372 (1982). https://doi.org/10.1007/BF00272859
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00272859