Summary
Ribosomal proteins from rat liver were separated by chromatography on carboxymethyl-cellulose into 9 fractions, which differ significantly in their electrophoretic pattern on polyacrylamide gel. Two of the fractions are homogeneous on examination by electrophoresis, two others contain a main component and several weak bands. Another protein component was isolated by filtration of a chromatographic fraction on Biogel P 10. Amino acid composition, N-terminal amino acids and molecular weights of the proteins were determined.
The combination of ion exchange chromatography and disc electrophoresis shows, that some chemically distinct proteins have almost identical electrophoretic mobility and other ones possess the same binding properties to CM-cellulose.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bielka, H., and H. Welfle: Characterization of ribosomal proteins from different tissues and species of animals by electrophoresis on polyacrylamide gel. Molec. Gen. Genetics 102, 128 (1968).
—, M. Böttger u. W. Förster unter Mitarbeit von I. Schneiders u. A. Henske: Strukturveränderungen und Dissoziation von Leberribosomen in Abhängigkeit von der Mg++-Konzentration. Europ. J. Biochem. 5, 183 (1968).
Chersi, A., M. Dzionara, D. Donner, and H. G. Wittmann: Ribosomal proteins. Molec. Gen. Genetics 101, 82 (1968).
Curry, J. B., and R. T. Hersh: Molecular weight of the protein from bovine liver ribosomes. Biochem. biophys. Res. Commun. 6, 415 (1962).
Elias, H. C.: Theorie und Praxis der Ultrazentrifugationstechnik. München: Beckman Instruments 1961.
Fogel, S., and P. S. Sypherd: Extraction and isolation of individual ribosomal proteins from E. coli. J. Bact. 96, 358 (1968).
Hamilton, M. G., and M. E. Ruth: Characterization of some of the proteins of the large subunit of rat liver ribosomes. Biochemistry 6, 2585 (1967).
Horstmann, H.-J.: Untersuchungen über Ribosomen-Proteine. I. Fraktionierung der ribosomalen Proteine der Bäckerhefe durch präparative Elektrophorese in Polyacrylamidgel. Hoppe-Seylers Z. physiol. Chem. 349, 405 (1968).
Kaltschmidt, E., M. Dzionara, D. Donner, and H. G. Wittmann: Ribosomal proteins. I. Isolation, amino acid composition, molecular weights and peptide mapping of proteins from E. coli ribosomes. Molec. Gen. Genetics 100, 364 (1967).
Leboy, P. S., E. C. Cox, and J. G. Flaks: The chromosomal site specifying a ribosomal protein in Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 52, 1367 (1964).
Moore, P. B., R. R. Traut, H. Noller, P. Pearson, and H. Delius: Ribosomal proteins of E. coli. II. Proteins of the 30 S subunit. J. molec. Biol. 31, 441 (1968).
Otaka, E., T. Itoh, and S. Osawa: Ribosomal proteins of bacterial cells: strain- and species-specifity. J. molec. Biol. 33, 93 (1968).
Schmidt, J., and B. R. Reid: Fractionation of yeast ribosomal proteins. Biochem. biophys. Res. Commun. 31, 654 (1968).
Stöffler, G., V. Rudloff, and H. G. Wittmann: Ribosomal proteins. III. Preparative separation of E. coli and yeast ribosomal proteins by means of gel filtration. Molec. Gen. Genetics 101, 70 (1968).
Tashiro, Y., and P. Siekevitz: Ultracentrifugal studies on the dissociation of hepatic ribosomes. J. molec. Biol. 11, 149 (1965).
Traut, R. R., R. B. Moore, H. Delius, H. Noller, and A. Tissieres: Ribosomal proteins of Escherichia coli. I. Demonstrations of different primary structures. Proc. nat. Acad. Sci. (Wash.) 57, 1294 (1967).
Wallenfels, K., u. A. Arens: Die Aminosäuren der Alkoholdehydrogenase aus Hefe. Biochem. Z. 332, 217 (1960).
Waller, J. P.: Fractionation of the ribosomal proteins from Escherichia coli. J. molec. Biol. 10, 319 (1964).
Welfle, H., u. H. Bielka: Charakterisierung von Proteinen aus Leber- und Hepatomribosomen durch Polyacrylamid-Gelelektrophorese. Z. Naturforsch. 23b, 690 (1968).
—, and M. Böttger: Studies on proteins of animal ribosomes. II. Separation of ribosomal proteins from rat liver by preparative polyacrylamide gel electrophoresis and some properties of the protein fractions. Molec. Gen. Genetics 104, 165 (1969).
Yphantis, D. A.: Rapid determination of molecular weights of peptides and proteins. Ann. N.Y. Acad. Sci. (Wash.) 88, 586 (1960).
Author information
Authors and Affiliations
Additional information
Communicated by H. G. Wittmann
Rights and permissions
About this article
Cite this article
Westermann, P., Bielka, H. & Böttger, M. Studies on proteins of animal ribosomes. Molec. Gen. Genet. 104, 157–164 (1969). https://doi.org/10.1007/BF00272797
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00272797