Skip to main content
SpringerLink
Log in

Protection and host cell repair of irradiated lambda phage

II. Irradiation of 5-bromouracil-substituted phage with near visible light

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Phage λ, in which thymine has been totally substituted by the analog, 5-bromouracil, have been irradiated with near visible light in the presence and absence of cysteamine, a free radical scavenger and proton donor. These phage were then assayed on normal bacteria and on mutants which are unable to repair DNA lesions. It is concluded that these phage suffer three different kinds of lesions: (1) a class which are not reparable, but which can prevented cysteamine, suggesting that they are sugar damages; (2) a class which are reparable but are not eliminated by cysteamine and (3) a class which can be neither eliminated nor repaired. The nature of the latter two types of lesions cannot be surmised at present.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Appleyard, R. K.: Segregation of new lysogenic types during growth of a doubly lysogenic strain derived from Escherichia coli K 12. Genetics 39, 440 (1954).

    Google Scholar 

  • — F. J. McGregor, and K. M. Baird: Mutation to extended host range and the occurrence of phenotypic mixing in the temperate coliphage lambda. Virology 2, 565 (1956).

    Google Scholar 

  • Bacq, Z., and P. Alexander: Fundamentals of radiobiology. New York: Pergamon Press 1961.

    Google Scholar 

  • Echols, H., and R. Gingery: Mutants of bacteriophage λ defective in vegetative genetic recombination. J. molec. Biol. 34, 239 (1968)

    Google Scholar 

  • Fox, E., and M. Meselson: Unegual photosensitivity of the two strands of DNA in bacteriophage λ. J. molec. Biol. 1, 583 (1963).

    Google Scholar 

  • Hotz, G., and H. Reuschl: Damage to deoxyribose molecules and to U-gene reactivation in UV-irradiated 5-bromouracil-DNA of phage T 4 Bor as influenced by cysteamine. Molec. Gen. Genetics 99, 5 (1967).

    Google Scholar 

  • Howard-Flanders, P.: DNA repair. Ann. Rev. Biochem. 37, 175 (1968).

    Google Scholar 

  • —, R. P., Boyce, and L. Theriot: Mechanism of sensitization of ultra-violet light of T 1 bacteriophage by incorporation of 5-bromodeoxyuridine or by pre-irradiation. Nature (Lond.) 195, 51 (1962).

    Google Scholar 

  • Hutchinson, F., and H. B. Hales: Effect of ultraviolet light on DNA which has been labeled with bromouracil. Progress Report, Yale University 1967.

  • Inman, R. B.: Denaturation maps of the left and right sides of the lambda DNA molecule determined by electron microscopy. J. molec. Biol. 28, 103 (1967).

    Google Scholar 

  • Koehnlein, W., and F. Hutchinson: ESR studies of normal and bromouracil substituted transforming DNA of Bacillus subtilis after irradiation with ultraviolet light. 3rd Int. Congr. Radiation Res., Cortina d'Ampezzo 1966.

  • Ormerod, M. G., and P. Alexander: On the mechanism of radiation protection by cysteamine: an investigation by means of ESR. Radiat. Res. 18, 495 (1963).

    Google Scholar 

  • Radman, M., A. Roller, and M. Errera: Protection and host cell repair of irradiated lambda phage. I. Irradiation of normal phage with ultraviolet light. Molec. Gen. Genetics 104 (1969).

  • Sauerbier, W.: The influence of 5-bromodeoxyuridine substitution on UV-sensitivity, host-cell reactivation and photo-reactivation in T 1 and P 22 H 5. Virology 15, 465 (1961).

    Google Scholar 

  • Signer, E. R., and J. Well: Recombination in bacteriophage λ. I. Mutants deficient in general recombination. J. molec. Biol. 34, 261 (1968).

    Google Scholar 

  • Smith, K. C.: The photochemistry of thymine and bromouracil in vivo. Photochem. Photobiol. 3, 1 (1964).

    Google Scholar 

  • Stahl, I. W., J. M. Crosemen, L. Okun, E. Fox, and C. Laird: Radiation sensitivity of bacteriophage containing 5-bromodeoxyuridine. Virology 13, 98 (1961).

    Google Scholar 

  • Wacker, A., H. D., Mennigmann, and N. Szybalski: Effect of “visible” light on 5-bromouracil-labelled DNA. Nature (Lond.) 196, 685 (1962).

    Google Scholar 

  • Weiss, B., and C. C. Richardson: Enzymatic breakage and joining of deoxyribonucleic acid. I. Repair of single-stranded breaks in DNA by an enzyme system from Escherichia coli infected with T 4 bacteriophage. Proc. nat. Acad. Sci. (Wash.) 57, 1021 (1967).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Biophysique et Readiobiologie, Faculté des Sciences, Université libre de Bruxelles, Bruxelles, Belgium

    M. Radman (Research fellow of European Molecular Biology Organization), A. Roller & M. Errera

Authors
  1. M. Radman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Roller
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Errera
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by P. Slonimski

ON leave of absence from Biochemistry Department, College of Physicians and Surgeons Columbia University, New York City, New York

Rights and permissions

Reprints and permissions

About this article

Cite this article

Radman, M., Roller, A. & Errera, M. Protection and host cell repair of irradiated lambda phage. Molec. Gen. Genet. 104, 147–151 (1969). https://doi.org/10.1007/BF00272795

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00272795

Keywords

Search

Navigation