Summary
Phage λ, irradiated with ultraviolet light in the presence and absence of the free-radical scavenger and proton donor, cysteamine, have been assayed on normal and DNA-repair deficient bacteria. It has, been concluded that cysteamine protects the phage against lesions of a type which can be repaired by the bacterial DNA-repair systems. Presumably, these are pyrimidine dimers.
Similar content being viewed by others
References
Bacq, Z., and P. Alexander: Fundamentals of Radiology. New York: Pergamon Press 1961.
Boyce, R. P., and P. Howard-Flanders: Release of ultraviolet light-induced thymine from DNA in E. coli K12. Proc. nat. Acad. Sci. (Wash.) 51, 293, (1964).
Clark, A. J., M. Chamberlin, R. P. Boyce, and P. Howard-Flanders: Abnormal metabolic response to ultraviolet light of a recombination deficient mutant of E. coli K12. J. molec. Biol. 19, 442 (1966).
Dunn, D. B., and J. D. Smith: Effects of 5-halogenated uracils on the growth of E. coli and their incorporation into deoxyribonucleic acids. Biochem. J. 67, 494 (1957).
Echols, H., and R. Gingery: Mutants of bacteriophage λ defective in vegetative genetic recombination. J. molec. Biol. 34, 239 (1968).
Gueron, M., G. Shulman, and J. Eisinger: Energy transfer in dinucleotides. Proc. nat. Acad. Sci. (Wash.) 56, 814 (1966).
Hotz, G.: Suppression by cysteamine of radiosensitization in 5-bromodeoxyuridine substituted phage T1. Biochem. biophys. Res. Commun. 11, 393 (1963).
Howard-Flanders, P.: DNA repair. Ann. Rev. Biochem. 37, 175 (1968).
—, R. P. Boyce, and L. Theriot: Three loci in E. coli K12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics 53, 119 (1966).
Kaiser, A. D.: A genetic study of the temperate coliphage λ. Virology 1, 424 (1955).
Latarjet, R., P. Morenne et R. Berger: Un appareil simple pour le dosage des rayonnements ultraviolets émis par les lampes germicides. Ann. Inst. Pasteur 85, 174 (1953).
Milvy, P., and I. Pullman: ESR studies of spin transfer in irradiated nucleic acid cysteamine systems. Radiat. Res. 34, 265 (1968).
Ormerod, M. G., and P. Alexander: On the mechanism of radiation protection by cysteamine: an investigation by means of ESR Radiat. Res. 18 495 (1963).
Putte, P. van de, C. A. van Sluis, van Dillewijn, and A. Rörsch: The location of genes controlling radiation sensitivity in E. coli. Mutation Res. 2, 97 (1965).
—, H. Zwenk, and A. Rörsch: Properties of four mutants of E. coli defective in genetic recombination. Mutation Res. 3, 381 (1966).
Setlow, R. B., and W. L. Carrier: The disappearance of thymine dimers from DNA: an error-correcting mechanism. Proc. nat. Acad. Sci. (Wash.) 51, 226 (1964).
Stern, M. G., and J. Blok: Influence of pH on radioprotection of E. coli by sulfhydril compounds. Int. J. Radiat. Biol. 13, 491 (1967).
Sussman, R., et F. Jacob: Sur un système de répression thermosensible chez le bactériophage λ d'E. coli. C.R. Acad. Sci. (Paris) 254, 1517 (1962).
Weigle, J. J., M. Meselson, and K. Paigen: Density alterations associated with transducing ability in bacteriophage lambda. J. molec. Biol. 1, 379 (1959).
Author information
Authors and Affiliations
Additional information
Communicated by P. Slonimski
On leave of absence from Biochemistry Department, College of Physicians and Surgeons Columbia University, New York City, New York.
Rights and permissions
About this article
Cite this article
Radman, M., Roller, A. & Errera, M. Protection and host cell repair of irradiated lambda phage. Molec. Gen. Genet. 104, 142–146 (1969). https://doi.org/10.1007/BF00272794
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00272794