Skip to main content
SpringerLink
Log in

In situ detection of early replication phases of a gemini virus in legume protoplasts

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Protoplasts of Phaseolus vulgaris L. (Top Crop), infected with bean golden mosaic virus, were isolated and fixed by various methods for in situ hybridization. An iodine-125 labeled probe was made from the replicative form of the virus. The localization and quantitation was done by autoradiography. Cell wall removal lowered the background and allowed a more accurate analysis. RNase was used to eliminate the possibility of hybrids to RNA. The evidence suggests a sequence of virus movements starting from rough endoplasm reticulum, moving to the nuclear membrane, and finally with the highest concentration inside the nucleus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BGMV:

bean golden mosaic virus

rfBGMV or rfDNA:

replicative double-stranded DNA virus

ssDNA:

single-stranded virus

References

  • Alfageme CR, Rudkin G, Cohen H (1976) Proc. Natl. Acad. Sci. (USA) 73:2038–2042

    Google Scholar 

  • Appels R, Steffensen DM, Craig S (1979) Exp. Cell Res. 124:436–441

    Google Scholar 

  • Coté, BD, Uhlenbeck DC, Steffensen DM (1980) Chromosoma 80:349–367

    Google Scholar 

  • Goodman RM (1977a) Nature 266:54–55

    Google Scholar 

  • Goodman RM (1977b) Virology 83:171–179

    Google Scholar 

  • Hafen E, Levine M, Garber RL, Gehring WJ (1983) EMBO Jour. 2:617–623

    Google Scholar 

  • Ikegami M, Haber S, Goodman RM (1981) Proc. Natl. Acad. Sci. (USA) 78:4102–4106

    Google Scholar 

  • Kim KS, Shock TL, Goodman RM (1978) Virology 89:22–33

    Google Scholar 

  • Nag A (1981) Ph.D. Dissertation. University of Illinois, Urbana

    Google Scholar 

  • Prensky W (1975) Methods in Cell Biol. 13:121–152.

    Google Scholar 

  • Rabin M, Uhlenbeck OC, Steffensen DM, Mangel WF (1984) J. Virol. 49:445–451.

    Google Scholar 

  • Reisman D, Ricciardi RP, Goodman RM (1979) Virology 97:388–395

    Google Scholar 

  • Symth DR, Shaw T (1979) Austral. J. Bot. 27:273–284

    Google Scholar 

  • Szabo P, Elder R, Steffensen DM, Uhlenbeck OC (1977) J. Mol. Biol. 115:539–562

    Google Scholar 

  • Velazquez JM, DiDomenico BJ, Lindquist S (1980) Cell 20:679–689

    Google Scholar 

  • Wimber DE, Duffey PA, Steffensen DM, Prensky W (1974) Chromosoma 47:353–359

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Genetics and Development, University of Illinois, 61801, Urbana, IL, USA

    Dale M. Steffensen

  2. Department of Biological Sciences, University of Alabama at Huntsville, 35899, Huntsville, AL, USA

    H. J. Wilson

  3. Calgene, Incorporated, 95616, Davis, CA, USA

    Robert M. Goodman

Authors
  1. Dale M. Steffensen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. J. Wilson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Robert M. Goodman
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by J.M. Widholm

Rights and permissions

Reprints and permissions

About this article

Cite this article

Steffensen, D.M., Wilson, H.J. & Goodman, R.M. In situ detection of early replication phases of a gemini virus in legume protoplasts. Plant Cell Reports 6, 462–465 (1987). https://doi.org/10.1007/BF00272783

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00272783

Keywords

Search

Navigation