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Extractive bioconversion of geraniol by a Vitis vinifera cell suspension employing a two-phase system

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Abstract

Among the problems associated with the bioconversion of monoterpenes by plant cell suspensions are the toxicity of some substrates and/or products at low concentrations, the transient state of nascent products and the length of time required to obtain the cell suspension. We investigated the extractive bioconversion of geraniol by a Vitis vinifera c.v. Muscat de Frontignan cell suspension in a two-phase system consisting of an aqueous nutrient phase surmounted by a lipid (Miglyol 812) phase. This system proved to be advantageous as it allowed a five-fold increase in the substrate load without causing any detrimental effect on the cell suspension, it improved the persistence of nascent products and it permitted the recycling or re-use of the cell suspension in a monoterpene bioconversion process.

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Author notes

  1. François Cormier

    Present address: Food Research Centre, Agriculture Canada, J2S 8E3, St-Hyacinthe, Quebec, Canada

Authors and Affiliations

  1. Laboratoire de Biotechnologie Végétale, Ecole Nationale Supérieure Agronomique, 145 Avenue de Muret, F-31076, Toulouse Cedex, France

    François Cormier & Christian Ambid

Authors
  1. François Cormier
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  2. Christian Ambid
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Additional information

Communicated by F. Constabel

CRASH contribution no. 060

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Cormier, F., Ambid, C. Extractive bioconversion of geraniol by a Vitis vinifera cell suspension employing a two-phase system. Plant Cell Reports 6, 427–430 (1987). https://doi.org/10.1007/BF00272774

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  • DOI: https://doi.org/10.1007/BF00272774

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